{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T19:49:42Z","timestamp":1782935382940,"version":"3.54.5"},"reference-count":62,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2016,11,1]],"date-time":"2016-11-01T00:00:00Z","timestamp":1477958400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A greater understanding of the molecular mechanisms that\n            <jats:named-content content-type=\"genus-species\">Borrelia burgdorferi<\/jats:named-content>\n            uses to survive during mammalian infection is critical for the development of novel diagnostic and therapeutic tools to improve the clinical management of Lyme disease. By use of an\n            <jats:italic>in vivo<\/jats:italic>\n            expression technology (IVET)-based approach to identify\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            genes expressed\n            <jats:italic>in vivo<\/jats:italic>\n            , we discovered the\n            <jats:italic>bb0318<\/jats:italic>\n            gene, which is thought to encode the ATPase component of a putative riboflavin ABC transport system. Riboflavin is a critical metabolite enabling all organisms to maintain redox homeostasis.\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            appears to lack the metabolic capacity for\n            <jats:italic>de novo<\/jats:italic>\n            synthesis of riboflavin and so likely relies on scavenging riboflavin from the host environment. In this study, we sought to investigate the role of\n            <jats:italic>bb0318<\/jats:italic>\n            in\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            pathogenesis. No\n            <jats:italic>in vitro<\/jats:italic>\n            growth defect was observed for the \u0394\n            <jats:italic>bb0318<\/jats:italic>\n            clone. However, the mutant spirochetes displayed reduced levels of survival when exposed to exogenous hydrogen peroxide or murine macrophages. Spirochetes lacking\n            <jats:italic>bb0318<\/jats:italic>\n            were found to have a 100-fold-higher 50% infectious dose than spirochetes containing\n            <jats:italic>bb0318<\/jats:italic>\n            . In addition, at a high inoculum dose,\n            <jats:italic>bb0318<\/jats:italic>\n            was found to be important for effective spirochete dissemination to deep tissues for as long as 3 weeks postinoculation and to be critical for\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            infection of mouse hearts. Together, these data implicate\n            <jats:italic>bb0318<\/jats:italic>\n            in the oxidative stress response of\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            and indicate the contribution of\n            <jats:italic>bb0318<\/jats:italic>\n            to\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            mammalian infectivity.\n          <\/jats:p>","DOI":"10.1128\/iai.00430-16","type":"journal-article","created":{"date-parts":[[2016,8,23]],"date-time":"2016-08-23T02:39:00Z","timestamp":1471919940000},"page":"3141-3151","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Gene\n            <i>bb0318<\/i>\n            Is Critical for the Oxidative Stress Response and Infectivity of Borrelia burgdorferi"],"prefix":"10.1128","volume":"84","author":[{"given":"Adrienne C.","family":"Showman","sequence":"first","affiliation":[{"name":"Division of Immunity and Pathogenesis, Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, Orlando, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Aranjuez","sequence":"additional","affiliation":[{"name":"Division of Immunity and Pathogenesis, Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, Orlando, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip P.","family":"Adams","sequence":"additional","affiliation":[{"name":"Division of Immunity and Pathogenesis, Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, Orlando, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3130-3795","authenticated-orcid":false,"given":"Mollie W.","family":"Jewett","sequence":"additional","affiliation":[{"name":"Division of Immunity and Pathogenesis, Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, Orlando, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/37551"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00817-13"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01698.x"},{"key":"e_1_3_3_5_2","first-page":"393","article-title":"Whole genome analyses of transporters in spirochetes: Borrelia burgdorferi and Treponema pallidum","volume":"2","author":"Saier MH","year":"2000","unstructured":"Saier MH, Jr, Paulsen IT. 2000. 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