{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T21:23:39Z","timestamp":1792185819453,"version":"4.4.0"},"reference-count":61,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2006,7,1]],"date-time":"2006-07-01T00:00:00Z","timestamp":1151712000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The addition of host-derived sialic acid to<jats:italic>Neisseria gonorrhoeae<\/jats:italic>lipooligosaccharide is hypothesized to be an important mechanism by which gonococci evade host innate defenses. This hypothesis is based primarily on in vitro assays of complement-mediated and phagocytic killing. Here we report that a nonpolar \u03b1-2,3-sialyltransferase (<jats:italic>lst<\/jats:italic>) mutant of<jats:italic>N. gonorrhoeae<\/jats:italic>was significantly attenuated in its capacity to colonize the lower genital tract of 17-\u03b2 estradiol-treated female BALB\/c mice during competitive infection with the wild-type strain. Genetic complementation of the<jats:italic>lst<\/jats:italic>mutation restored recovery of the mutant to wild-type levels. Studies with B10.D2-HC<jats:sup>o<\/jats:sup>H2<jats:sup>d<\/jats:sup>H<jats:sup>2<\/jats:sup>-T18c\/OSN (C5-deficient) mice showed that attenuation of the<jats:italic>lst<\/jats:italic>mutant was not due to increased sensitivity to complement-mediated bacteriolysis, a result that is consistent with recently reported host restrictions in the complement cascade. However, Lst-deficient gonococci were killed more rapidly than sialylated wild-type gonococci following intraperitoneal injection into normal mice, which is consistent with sialylation conferring protection against killing by polymorphonuclear leukocytes (PMNs). As reported for human PMNs, sialylated gonococci were more resistant to killing by murine PMNs, and sialylation led to reduced association with and induction of a weaker respiratory burst in PMNs from estradiol-treated mice. In summary, these studies suggest sialylation confers a survival advantage to<jats:italic>N. gonorrhoeae<\/jats:italic>in mice by increasing resistance to PMN killing. This report is the first direct demonstration that \u03b1-2,3-sialyltransferase contributes to<jats:italic>N. gonorrhoeae<\/jats:italic>pathogenesis in an in vivo model. This study also validates the use of experimental murine infection to study certain aspects of gonococcal pathogenesis.<\/jats:p>","DOI":"10.1128\/iai.00433-06","type":"journal-article","created":{"date-parts":[[2006,6,21]],"date-time":"2006-06-21T23:10:29Z","timestamp":1150931429000},"page":"4094-4103","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["\u03b1-2,3-Sialyltransferase Enhances<i>Neisseria gonorrhoeae<\/i>Survival during Experimental Murine Genital Tract Infection"],"prefix":"10.1128","volume":"74","author":[{"given":"Hong","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann E.","family":"Jerse","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/162.2.506"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01345.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(01)01496-4"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1432026100"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.4.1038-1049.2004"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4827(02)00010-1"},{"key":"e_1_3_2_8_2","first-page":"1204","volume":"53","year":"2005","unstructured":"Centers for Disease Control and Prevention. 2005. 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