{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:11:34Z","timestamp":1792627894906,"version":"4.5.0"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2016,10,17]],"date-time":"2016-10-17T00:00:00Z","timestamp":1476662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2016,11,1]],"date-time":"2016-11-01T00:00:00Z","timestamp":1477958400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Adherent\/invasive\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            (AIEC) strains have recently been receiving increased attention because they are more prevalent and persistent in the intestine of Crohn's disease (CD) patients than in healthy subjects. Since AIEC strains show a high percentage of similarity to extraintestinal pathogenic\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            (ExPEC), neonatal meningitis-associated\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            (NMEC), and uropathogenic\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            (UPEC) strains, here we compared AIEC strain LF82 with a UPEC isolate (strain EC73) to assess whether LF82 would be able to infect prostate cells as an extraintestinal target. The virulence phenotypes of both strains were determined by using the RWPE-1 prostate cell line. The results obtained indicated that LF82 and EC73 are able to adhere to, invade, and survive within prostate epithelial cells. Invasion was confirmed by immunofluorescence and electron microscopy. Moreover, cytochalasin D and colchicine strongly inhibited bacterial uptake of both strains, indicating the involvement of actin microfilaments and microtubules in host cell invasion. Moreover, both strains belong to phylogenetic group B2 and are strong biofilm producers.\n            <jats:italic>In silico<\/jats:italic>\n            analysis reveals that LF82 shares with UPEC strains several virulence factors: namely, type 1 pili, the group II capsule, the vacuolating autotransporter toxin, four iron uptake systems, and the pathogenic island (PAI). Furthermore, compared to EC73, LF82 induces in RWPE-1 cells a marked increase of phosphorylation of mitogen-activated protein kinases (MAPKs) and of NF-\u03baB already by 5 min postinfection, thus inducing a strong inflammatory response. Our\n            <jats:italic>in vitro<\/jats:italic>\n            data support the hypothesis that AIEC strains might play a role in prostatitis, and, by exploiting host-cell signaling pathways controlling the innate immune response, likely facilitate bacterial multiplication and dissemination within the male genitourinary tract.\n          <\/jats:p>","DOI":"10.1128\/iai.00438-16","type":"journal-article","created":{"date-parts":[[2016,9,7]],"date-time":"2016-09-07T01:30:00Z","timestamp":1473211800000},"page":"3105-3113","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["The Adherent\/Invasive Escherichia coli Strain LF82 Invades and Persists in Human Prostate Cell Line RWPE-1, Activating a Strong Inflammatory Response"],"prefix":"10.1128","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0753-6003","authenticated-orcid":false,"given":"Maria P.","family":"Conte","sequence":"first","affiliation":[{"name":"Department of Public Health and Infectious Diseases, Sapienza University, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Aleandri","sequence":"additional","affiliation":[{"name":"Department of Public Health and Infectious Diseases, Sapienza University, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimiliano","family":"Marazzato","sequence":"additional","affiliation":[{"name":"Department of Public Health and Infectious Diseases, Sapienza University, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonietta L.","family":"Conte","sequence":"additional","affiliation":[{"name":"Department of Public Health and Infectious Diseases, Sapienza University, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Ambrosi","sequence":"additional","affiliation":[{"name":"Department of Public Health and Infectious Diseases, Sapienza University, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauro","family":"Nicoletti","sequence":"additional","affiliation":[{"name":"Department of Medical, Oral and Biotechnological Sciences, G. D'Annunzio University, Chieti, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlo","family":"Zagaglia","sequence":"additional","affiliation":[{"name":"Department of Public Health and Infectious Diseases, Sapienza University, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guido","family":"Gambara","sequence":"additional","affiliation":[{"name":"Center of Space Medicine Berlin, Neuromuscular System, Institute of Anatomy, Charit\u00e9-Universit\u00e4tsmedizin Berlin, Berlin, Germany"},{"name":"Department of Anatomy, Histology, Forensic Medicine and Orthopedics, Unit of Histology and Medical Embryology, Sapienza University, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fioretta","family":"Palombi","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology, Forensic Medicine and Orthopedics, Unit of Histology and Medical Embryology, Sapienza University, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"De Cesaris","sequence":"additional","affiliation":[{"name":"Department of Biotechnological and Applied Clinical Sciences, University of L'Aquila, L'Aquila, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elio","family":"Ziparo","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Histology, Forensic Medicine and Orthopedics, Unit of Histology and Medical Embryology, Sapienza University, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna T.","family":"Palamara","sequence":"additional","affiliation":[{"name":"Department of Public Health and Infectious Diseases, Pasteur Institute Cenci Bolognetti Foundation, Sapienza University, Rome, Italy"},{"name":"IRCCS, San Raffaele Pisana, Rome, 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