{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T20:53:16Z","timestamp":1791060796536,"version":"4.1.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2007,10,1]],"date-time":"2007-10-01T00:00:00Z","timestamp":1191196800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The<jats:italic>Brucella abortus<\/jats:italic>two-component regulatory system BvrR\/BvrS controls the expression of outer membrane proteins (Omp) Omp3a (Omp25) and Omp3b (Omp22). Disruption of<jats:italic>bvrS<\/jats:italic>or<jats:italic>bvrR<\/jats:italic>generates avirulent mutants with altered cell permeability, higher sensitivity to microbicidal peptides, and complement. Consequently, the role of Omp3a and Omp3b in virulence was examined. Similar to<jats:italic>bvrS<\/jats:italic>or<jats:italic>bvrR<\/jats:italic>mutants,<jats:italic>omp3a<\/jats:italic>and<jats:italic>omp3b<\/jats:italic>mutants displayed increased attachment to cells, indicating surface alterations. However, they showed unaltered permeability; normal expression of Omp10, Omp16, Omp19, Omp2b, and Omp1; native hapten polysaccharide; and lipopolysaccharide and were resistant to complement and polymyxin B at ranges similar to those of the wild-type (WT) counterpart. Likewise,<jats:italic>omp3a<\/jats:italic>and<jats:italic>omp3b<\/jats:italic>mutants were able to replicate in murine macrophages and in HeLa cells, were resistant to the killing action of human neutrophils, and persisted in mice, like the WT strain. Murine macrophages infected with the<jats:italic>omp3a<\/jats:italic>mutant generated slightly higher levels of tumor necrosis factor alpha than the WT, whereas the<jats:italic>bvrS<\/jats:italic>mutant induced lower levels of this cytokine. Since the absence of Omp3a or Omp3b does not result in attenuation, it can be concluded that BvrR\/BvrS influences additional<jats:italic>Brucella<\/jats:italic>properties involved in virulence. Our results are discussed in the light of previous works suggesting that disruption of<jats:italic>omp3a<\/jats:italic>generates attenuated<jats:italic>Brucella<\/jats:italic>strains, and we speculate on the role of group 3 Omps.<\/jats:p>","DOI":"10.1128\/iai.00439-07","type":"journal-article","created":{"date-parts":[[2007,7,31]],"date-time":"2007-07-31T00:59:11Z","timestamp":1185843551000},"page":"4867-4874","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["BvrR\/BvrS-Controlled Outer Membrane Proteins Omp3a and Omp3b Are Not Essential for<i>Brucella abortus<\/i>Virulence"],"prefix":"10.1128","volume":"75","author":[{"given":"Lorea","family":"Manterola","sequence":"first","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Parasitologi\u0301a, Universidad de Navarra, 31008 Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Guzma\u0301n-Verri","sequence":"additional","affiliation":[{"name":"Programa de Investigacio\u0301n en Enfermedades Tropicales (PIET), Escuela de Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esteban","family":"Chaves-Olarte","sequence":"additional","affiliation":[{"name":"Programa de Investigacio\u0301n en Enfermedades Tropicales (PIET), Escuela de Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica"},{"name":"Centro de Investigacio\u0301n en Enfermedades Tropicales, Facultad de Microbiologi\u0301a, Universidad de Costa Rica, 1000 San Jose\u0301, Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eli\u0301as","family":"Barquero-Calvo","sequence":"additional","affiliation":[{"name":"Programa de Investigacio\u0301n en Enfermedades Tropicales (PIET), Escuela de Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari\u0301a-Jesu\u0301s","family":"de Miguel","sequence":"additional","affiliation":[{"name":"Centro de Investigacio\u0301n y Tecnologi\u0301a Agroalimentaria (CITA), Sanidad Animal, Gobierno de Arago\u0301n, Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Moriyo\u0301n","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Parasitologi\u0301a, Universidad de Navarra, 31008 Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari\u0301a-Jesu\u0301s","family":"Grillo\u0301","sequence":"additional","affiliation":[{"name":"Instituto de Agrobiotecnologi\u0301a, CSIC-UPNA-Gobierno de Navarra, Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Lo\u0301pez-Gon\u0303i","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Parasitologi\u0301a, Universidad de Navarra, 31008 Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgardo","family":"Moreno","sequence":"additional","affiliation":[{"name":"Programa de Investigacio\u0301n en Enfermedades Tropicales (PIET), Escuela de Medicina Veterinaria, Universidad Nacional, 304-3000 Heredia, Costa Rica"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Techniques for the brucellosis laboratory. 1988"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1202"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.10.5693-5703.2004"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00486-07"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"2885","DOI":"10.4049\/jimmunol.156.8.2885","volume":"156","year":"1996","unstructured":"Caron, E., A. Gross, J. P. Liautard, and J. Dornand. 1996. Brucella species release a specific, protease-sensitive, inhibitor of TNF-alpha expression, active on human macrophage-like cells. J. Immunol.156:2885-2893.","journal-title":"J. Immunol."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030088"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2002.00221.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.01.001"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.6.2047-2055.1996"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00211-0"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2005.11.008"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00223-7"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.2144\/96214bm07"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.2001.62.1461"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00110-4"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1053\/rvsc.2002.0555"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00502.x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.55.3.609-615.1987"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1993.tb06439.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.4.1309-1316.1998"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M105606200"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.192439399"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.8.2715-2726.2005"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.8.4823-4830.2001"},{"key":"e_1_3_2_26_2","first-page":"800","volume":"16","year":"1994","unstructured":"Kovach, M. E., R. W. Phillips, P. H. Elzer, R. M. Roop II, and K. M. Peterson. 1994. pBBR1MCS: a broad-host-range cloning vector. BioTechniques16:800-802.","journal-title":"BioTechniques"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr060636a"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2004.12.003"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.16.5631-5639.2005"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.16.5273-5275.1993"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.31.1.362-370.1981"},{"key":"e_1_3_2_32_2","first-page":"287","year":"2004","unstructured":"Moreno, E., and J.-P. Gorvel. 2004. Invasion, intracellular trafficking and replication of Brucella organisms in professional and non-professional phagocytes, p.287-312. In I. L\u00f3pez-Go\u00f1i and I. Moriy\u00f3n (ed.), Brucella: molecular and cellular biology. Horizon Bioscience, Norfolk, United Kingdom.","journal-title":"Brucella: molecular and cellular biology."},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.022622699"},{"key":"e_1_3_2_34_2","first-page":"315","volume":"5","year":"2006","unstructured":"Moreno, E., and I. Moriy\u00f3n. 2006. The genus Brucella, p.315-456. In M. Dorkin, S. Falkow, E. Rosenberg, K.-H. Schleifer, and E. Stackebrandt (ed.), The prokaryotes,vol. 5. Springer-Verlag, New York, NY.","journal-title":"The prokaryotes"},{"key":"e_1_3_2_35_2","first-page":"19","volume":"1","year":"1998","unstructured":"Moriy\u00f3n, I., and I. L\u00f3pez-Go\u00f1i. 1998. Structure and properties of the outer membranes of Brucella abortus and Brucella melitensis. Int. Microbiol.1:19-26.","journal-title":"Int. Microbiol."},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.12.5711-5724.1998"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.2.489-494.1990"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.8.4326-4332.2003"},{"key":"e_1_3_2_39_2","unstructured":"Molecular cloning: a laboratory manual 1989"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/0882-4010(91)90040-H"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1183-784"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01285.x"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00913.x"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.10.5540-5546.2002"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.35.3.979-989.1982"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2004.04.009"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-143-9-2913"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.8.5137-5143.2005"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00439-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00439-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,13]],"date-time":"2023-05-13T16:24:42Z","timestamp":1683995082000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00439-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,10]]},"references-count":47,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2007,10]]}},"alternative-id":["10.1128\/IAI.00439-07"],"URL":"https:\/\/doi.org\/10.1128\/iai.00439-07","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,10]]},"assertion":[{"value":"2007-03-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-07-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}