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Phenotypic analysis of an isogenic<jats:italic>mntH<\/jats:italic>mutant indicates that MntH is the sole high-affinity manganese transporter in this bacterium but that MntH does not play a detectable role in the transport of Fe<jats:sup>2+<\/jats:sup>, Zn<jats:sup>2+<\/jats:sup>, Co<jats:sup>2+<\/jats:sup>, or Ni<jats:sup>2+<\/jats:sup>. Consistent with the apparent selectivity of the corresponding gene product, the expression of the<jats:italic>mntH<\/jats:italic>gene in<jats:italic>B. abortus<\/jats:italic>2308 is repressed by Mn<jats:sup>2+<\/jats:sup>, but not Fe<jats:sup>2+<\/jats:sup>, and this Mn-responsive expression is mediated by a Mur-like repressor. The<jats:italic>B. abortus mntH<\/jats:italic>mutant MWV15 exhibits increased susceptibility to oxidative killing in vitro compared to strain 2308, and a comparative analysis of the superoxide dismutase activities present in these two strains indicates that the parental strain requires MntH in order to make wild-type levels of its manganese superoxide dismutase SodA. The<jats:italic>B. abortus mntH<\/jats:italic>mutant also exhibits extreme attenuation in both cultured murine macrophages and experimentally infected C57BL\/6 mice. These experimental findings indicate that Mn<jats:sup>2+<\/jats:sup>transport mediated by MntH plays an important role in the physiology of<jats:italic>B. abortus<\/jats:italic>2308, particularly during its intracellular survival and replication in the host.<\/jats:p>","DOI":"10.1128\/iai.00444-09","type":"journal-article","created":{"date-parts":[[2009,6,2]],"date-time":"2009-06-02T00:58:56Z","timestamp":1243904336000},"page":"3466-3474","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":88,"title":["The Manganese Transporter MntH Is a Critical Virulence Determinant for<i>Brucella abortus<\/i>2308 in Experimentally Infected Mice"],"prefix":"10.1128","volume":"77","author":[{"given":"Eric S.","family":"Anderson","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, North Carolina 27834"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James T.","family":"Paulley","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, North Carolina 27834"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer M.","family":"Gaines","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, North Carolina 27834"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle W.","family":"Valderas","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, Oklahoma State University Center for Veterinary Health Sciences, Stillwater, Oklahoma 74078"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel W.","family":"Martin","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, North Carolina 27834"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evan","family":"Menscher","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, North Carolina 27834"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy D.","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, North Carolina 27834"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin S.","family":"Burns","sequence":"additional","affiliation":[{"name":"Department of Chemistry, East Carolina University, Greenville, North Carolina 27858"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"II","given":"R. 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