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Within host cells,\n              <jats:italic>C. burnetii<\/jats:italic>\n              proliferates in a spacious, acidic, lysosome-derived\n              <jats:italic>Coxiella<\/jats:italic>\n              -containing vacuole (CCV) by a process that requires the Dot\/Icm type IVB secretion system to deliver effectors that manipulate host cell functions. A previous transposon mutagenesis screen identified the gene\n              <jats:italic>cbu0937<\/jats:italic>\n              as being important for intracellular replication of\n              <jats:italic>C. burnetii<\/jats:italic>\n              . Here, the function of Cbu0937 was investigated. The\n              <jats:italic>cbu0937<\/jats:italic>\n              ::Tn mutant had no detectable defect replicating in the axenic acidified citrate cysteine medium 2. Additionally, intracellular replication of the\n              <jats:italic>cbu0937<\/jats:italic>\n              ::Tn mutant was not restored by co-infection of host cells with an isogenic wild-type strain of\n              <jats:italic>C. burnetii<\/jats:italic>\n              . Thus, the\n              <jats:italic>cbu0937<\/jats:italic>\n              ::Tn mutant has a cell-intrinsic intracellular replication defect. Intracellular replication of the\n              <jats:italic>cbu0937<\/jats:italic>\n              ::Tn mutant was restored by complementing the gene\n              <jats:italic>in trans<\/jats:italic>\n              with a plasmid encoding either untagged or an epitope-tagged version of Cbu0937. Analysis of the predicted structure of the Cbu0937 protein using AlphaFold revealed high similarity between Cbu0937 and several bacterial porins. Fractionation studies and surface labeling of\n              <jats:italic>C. burnetii<\/jats:italic>\n              producing a functional epitope-tagged protein indicated the localization of Cbu0937 to the bacterial outer membrane. From these data, we conclude that\n              <jats:italic>cbu0937<\/jats:italic>\n              encodes a porin that plays an essential role in supporting\n              <jats:italic>C. burnetii<\/jats:italic>\n              intracellular replication, which likely involves the acquisition of an important metabolite in the CCV lumen.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/iai.00448-24","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T13:02:41Z","timestamp":1741784561000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Identification of a\n            <i>Coxiella burnetii<\/i>\n            outer membrane porin required for intracellular 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