{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T20:29:46Z","timestamp":1758400186540,"version":"3.37.3"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2016,10,1]],"date-time":"2016-10-01T00:00:00Z","timestamp":1475280000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["3031-2010-RGPIN"],"award-info":[{"award-number":["3031-2010-RGPIN"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Tonsils conduct immune surveillance of antigens entering the upper respiratory tract. Despite their immunological function, they are also sites of persistence and invasion of bacterial pathogens.\n            <jats:named-content content-type=\"genus-species\">Actinobacillus suis<\/jats:named-content>\n            is a common resident of the tonsils of the soft palate in pigs, but under certain circumstances it can invade, causing septicemia and related sequelae. Twenty-four putative adhesins are predicted in the\n            <jats:named-content content-type=\"genus-species\">A. suis<\/jats:named-content>\n            genome, but to date, little is known about how they might participate in colonization or invasion. To better understand these processes, swine tonsil lysates were characterized by mass spectrometry. Fifty-nine extracellular matrix (ECM) proteins were identified, including small leucine-rich proteoglycans, integrins, and other cell surface receptors. Additionally, attachment of the wild type and 3 adhesin mutants to 5 ECM components was evaluated. Exponential cultures of wild-type\n            <jats:named-content content-type=\"genus-species\">A. suis<\/jats:named-content>\n            adhered significantly more than stationary cultures to all ECM components studied except collagen I. During exponential growth, the\n            <jats:named-content content-type=\"genus-species\">A. suis<\/jats:named-content>\n            \u0394\n            <jats:italic>flp1<\/jats:italic>\n            mutant attached less to collagen IV while the \u0394\n            <jats:italic>ompA<\/jats:italic>\n            mutant attached less to all ECMs. The \u0394\n            <jats:italic>comE1<\/jats:italic>\n            strain attached less to collagen IV, fibronectin, and vitronectin during exponential growth and exhibited differential attachment to collagen I over short adherence time points. These results suggest that Flp1, OmpA, and ComE1 are important during early stages of attachment to ECM components found in tonsils, which supports the notion that other adhesins have compensatory effects during later stages of attachment.\n          <\/jats:p>","DOI":"10.1128\/iai.00456-16","type":"journal-article","created":{"date-parts":[[2016,8,2]],"date-time":"2016-08-02T03:05:13Z","timestamp":1470107113000},"page":"2944-2952","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Attachment of Actinobacillus suis H91-0380 and Its Isogenic Adhesin Mutants to Extracellular Matrix Components of the Tonsils of the Soft Palate of Swine"],"prefix":"10.1128","volume":"84","author":[{"given":"Adina R.","family":"Bujold","sequence":"first","affiliation":[{"name":"Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janet I.","family":"MacInnes","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/472460"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1079\/AHRR200358"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0185(199605)245:1<102::AID-AR15>3.0.CO;2-T"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/S1466252312000102"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.5.2532"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711760410"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.4A6253.2004"},{"key":"e_1_3_3_9_2","first-page":"270","article-title":"Extracellular matrix proteins and integrin receptors in reactive and non-reactive lymph nodes","volume":"86","author":"Casta\u00f1os-Velez E","year":"1995","unstructured":"Casta\u00f1os-Velez E, Biberfeld P, Patarroyo M. 1995. Extracellular matrix proteins and integrin receptors in reactive and non-reactive lymph nodes. Immunology 86:270\u2013278.","journal-title":"Immunology"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2015.11.001"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/1755-1536-4-21"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07373.x"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-16555-9"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-16555-9"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003057"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.16.1.301"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00819-08"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/005892-0"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiv374"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-014-9601-6"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.00830.x"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003991"},{"key":"e_1_3_3_23_2","first-page":"242","article-title":"Prevalence of Actinobacillus pleuropneumoniae, Actinobacillus suis, Haemophilus parasuis, Pasteurella multocida, and Streptococcus suis in representative Ontario swine herds","volume":"72","author":"MacInnes JI","year":"2008","unstructured":"MacInnes JI, Gottschalk M, Lone AG, Metcalf DS, Ojha S, Rosendal T, Watson SB, Friendship RM. 2008. Prevalence of Actinobacillus pleuropneumoniae, Actinobacillus suis, Haemophilus parasuis, Pasteurella multocida, and Streptococcus suis in representative Ontario swine herds. Can J Vet Res 72:242\u2013248.","journal-title":"Can J Vet Res"},{"key":"e_1_3_3_24_2","first-page":"83","article-title":"Agents of the \u201csuis-ide diseases\u201d of swine: Actinobacillus suis, Haemophilus parasuis, and Streptococcus suis","volume":"63","author":"MacInnes JI","year":"1999","unstructured":"MacInnes JI, Desrosiers R. 1999. Agents of the \u201csuis-ide diseases\u201d of swine: Actinobacillus suis, Haemophilus parasuis, and Streptococcus suis. Can J Vet Res 63:83\u201389.","journal-title":"Can J Vet Res"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.10.7032-7039.2005"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.07.014"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-015-1659-x"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1636"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01633-12"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.5.1131-1137.1997"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(03)00064-8"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13104-015-1045-8"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1999.tb08721.x"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.4267\/2042\/37719"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.4267\/2042\/44402"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2003.11.006"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.2209\/tdcpublication.51.145"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01347-07"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00312-08"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01349-07"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(91)90096-E"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00456-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00456-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T10:06:07Z","timestamp":1646474767000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00456-16"}},"subtitle":[],"editor":[{"given":"G. H.","family":"Palmer","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2016,10]]},"references-count":40,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2016,10]]}},"alternative-id":["10.1128\/IAI.00456-16"],"URL":"https:\/\/doi.org\/10.1128\/iai.00456-16","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"type":"print","value":"0019-9567"},{"type":"electronic","value":"1098-5522"}],"subject":[],"published":{"date-parts":[[2016,10]]},"assertion":[{"value":"2016-05-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-07-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-09-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}