{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T13:52:51Z","timestamp":1792072371444,"version":"4.4.0"},"reference-count":35,"publisher":"American Society for Microbiology","license":[{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:00:00Z","timestamp":1792022400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K22AI153677"],"award-info":[{"award-number":["K22AI153677"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI166535"],"award-info":[{"award-number":["R01AI166535"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35GM154838"],"award-info":[{"award-number":["R35GM154838"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R35GM154857"],"award-info":[{"award-number":["R35GM154857"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["NIFA-AFRI-2019-67017-29261"],"award-info":[{"award-number":["NIFA-AFRI-2019-67017-29261"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008893","name":"University of Iowa","doi-asserted-by":"publisher","award":["Start Up"],"award-info":[{"award-number":["Start Up"]}],"id":[{"id":"10.13039\/100008893","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014455","name":"University of Tennessee","doi-asserted-by":"publisher","award":["Start up and Human Health and Wellness Initiative Funds"],"award-info":[{"award-number":["Start up and Human Health and Wellness Initiative Funds"]}],"id":[{"id":"10.13039\/100014455","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Carver College of Medicine Stead Family Innovation Scholars Program","award":["Stead Family Innovation Scholars Program"],"award-info":[{"award-number":["Stead Family Innovation Scholars Program"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Previous research demonstrated that\n                      <jats:italic toggle=\"yes\">Campylobacter jejuni<\/jats:italic>\n                      encodes a heme utilization system that facilitates heme-dependent growth under iron-limiting conditions, and that transcription of this system is induced during human infection. Despite these observations, it remained unknown whether the heme transport system is required for colonization and disease in a susceptible host. To address this, we created individual non-polar deletion mutants of each component of the heme transport system and examined their ability to promote heme-dependent growth and iron uptake. From this work, we found that only the heme receptor, ChuA, was required for heme-dependent growth and iron acquisition, which supports the earlier work of another group. Furthermore, we examined whether intestinal colonization, immune activation, and pathology were altered during infection with these mutants. After establishing that elevated heme and\n                      <jats:italic toggle=\"yes\">chuABCD<\/jats:italic>\n                      expression occur during\n                      <jats:italic toggle=\"yes\">C. jejuni<\/jats:italic>\n                      infection of IL-10\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      mice, we found that a mutant of the heme receptor, ChuA, exhibited significantly reduced colonization of the colon. In addition, we found that neutrophil and circulating monocyte recruitment were significantly reduced in the colon during infection with the ChuA mutant, but that populations of self-maintaining tissue macrophages remained high. Loss of ChuA reduced colonic colonization and was accompanied by diminished innate immune cell recruitment and intestinal pathology. Together, these findings identify ChuA-dependent heme acquisition as a key determinant of efficient colonic colonization and the associated inflammatory disease.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/iai.00457-26","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T13:38:12Z","timestamp":1792071492000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Efficient colonic colonization by\n                    <i>Campylobacter jejuni<\/i>\n                    requires the heme receptor ChuA"],"prefix":"10.1128","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-7231-5150","authenticated-orcid":true,"given":"Binita","family":"Baral","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036jqmy94","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, University of Iowa","place":["Iowa City, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5771-1549","authenticated-orcid":true,"given":"Madison L.","family":"Bunch","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036jqmy94","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, University of Iowa","place":["Iowa City, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily L.","family":"Roberts","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036jqmy94","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, University of Iowa","place":["Iowa City, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent R.","family":"Randaisi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/020f3ap87","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, University of Tennessee","place":["Knoxville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wyatt W.","family":"Wittliff","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05ect4e57","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University and Agricultural and Mechanical College","place":["Baton Rouge, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0285-7775","authenticated-orcid":true,"given":"William N.","family":"Beavers","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05ect4e57","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University and Agricultural and Mechanical College","place":["Baton Rouge, USA"]},{"id":[{"id":"https:\/\/ror.org\/05ect4e57","id-type":"ROR","asserted-by":"publisher"}],"name":"Mass Spectrometry Resource Center, Louisiana State University School of Veterinary Medicine","place":["Baton Rouge, USA"]},{"id":[{"id":"https:\/\/ror.org\/05ect4e57","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Veterinary Medicine, Louisiana State University and Agricultural and Mechanical College","place":["Baton Rouge, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7259-6574","authenticated-orcid":true,"given":"Andrew J.","family":"Monteith","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/020f3ap87","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology, University of Tennessee","place":["Knoxville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1552-3253","authenticated-orcid":true,"given":"David K.","family":"Meyerholz","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036jqmy94","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pathology, University of Iowa","place":["Iowa City, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5302-3247","authenticated-orcid":true,"given":"Jeremiah G.","family":"Johnson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036jqmy94","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, University of Iowa","place":["Iowa City, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","published-online":{"date-parts":[[2026,10,15]]},"reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2019.e02814"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X.JFP-11-058"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm7326a1"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.3.1363-1364.2004"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00116-21"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01021-09"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-024-09709-y"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0037-9"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom13030449"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/852195"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kem339"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1012797"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2836"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2013.00080"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.04.010"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00994-06"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-018-0133-7"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1118321109"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2013.00075"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2021.100275"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.4161\/gmic.1.1.10951"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2017-0295"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1039\/c3mt20254b"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2020.100029"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-025-02124-y"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1186\/2050-7771-2-1"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/cti2.70111"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2020-323121"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2010.66"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells9061456"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1279826"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000949"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0073586"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.633495"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41374-018-0057-0"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.00457-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.00457-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T13:38:14Z","timestamp":1792071494000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/iai.00457-26"}},"subtitle":[],"editor":[{"given":"Alison K.","family":"Criss","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,10,15]]},"references-count":35,"alternative-id":["10.1128\/iai.00457-26"],"URL":"https:\/\/doi.org\/10.1128\/iai.00457-26","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,15]]},"assertion":[{"value":"2026-07-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00457-26"}}