{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T09:00:45Z","timestamp":1790413245299,"version":"4.1.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2009,8,1]],"date-time":"2009-08-01T00:00:00Z","timestamp":1249084800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2009,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Coccidioidomycosis (also known as San Joaquin Valley fever) is an occupational disease. Workers exposed to outdoor dust which contains spores of the soil-inhabiting fungus have a significantly increased risk of respiratory infection. In addition, people with compromised T-cell immunity, the elderly, and certain racial groups, particularly African-Americans and Filipinos, who live in regions of endemicity in the southwestern United States have an elevated incidence of symptomatic infection caused by inhalation of spores of\n                    <jats:italic>Coccidioides posadasii<\/jats:italic>\n                    or\n                    <jats:italic>Coccidioides immitis<\/jats:italic>\n                    . Recurring epidemics and escalation of medical costs have helped to motivate production of a vaccine against valley fever. The major focus has been the development of a defined, T-cell-reactive, recombinant protein vaccine. However, none of the products described to date have provided full protection to coccidioidal disease-susceptible BALB\/c mice. Here we describe the first genetically engineered, live, attenuated vaccine that protects both BALB\/c and C57BL\/6 mice against coccidioidomycosis. Two chitinase genes (\n                    <jats:italic>CTS2<\/jats:italic>\n                    and\n                    <jats:italic>CTS3<\/jats:italic>\n                    ) were disrupted to yield the attenuated strain, which was unable to endosporulate and was no longer infectious. Vaccinated survivors mounted an immune response characterized by production of both T-helper-1- and T-helper-2-type cytokines. Histology revealed well-formed granulomas and markedly diminished inflammation. Significantly fewer organisms were observed in the lungs of survivors than in those of nonvaccinated mice. Additional investigations are required to further define the nature of the live, attenuated vaccine-induced immunity against\n                    <jats:italic>Coccidioides<\/jats:italic>\n                    infection.\n                  <\/jats:p>","DOI":"10.1128\/iai.00459-09","type":"journal-article","created":{"date-parts":[[2009,6,1]],"date-time":"2009-06-01T20:58:56Z","timestamp":1243889936000},"page":"3196-3208","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":103,"title":["A Genetically Engineered Live Attenuated Vaccine of\n                    <i>Coccidioides posadasii<\/i>\n                    Protects BALB\/c Mice against Coccidioidomycosis"],"prefix":"10.1128","volume":"77","author":[{"given":"Jianmin","family":"Xue","sequence":"first","affiliation":[{"name":"Department of Biology and South Texas Center for Emerging Infectious Diseases, University of Texas, San Antonio, Texas 78249"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Biology and South Texas Center for Emerging Infectious Diseases, University of Texas, San Antonio, Texas 78249"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dale","family":"Selby","sequence":"additional","affiliation":[{"name":"Department of Pathology, Wilford Hall Medical Center, Lackland Air Force Base, Texas 78236"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiung-Yu","family":"Hung","sequence":"additional","affiliation":[{"name":"Department of Biology and South Texas Center for Emerging Infectious Diseases, University of Texas, San Antonio, Texas 78249"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jieh-Juen","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Biology and South Texas Center for Emerging Infectious Diseases, University of Texas, San Antonio, Texas 78249"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garry T.","family":"Cole","sequence":"additional","affiliation":[{"name":"Department of Biology and South Texas Center for Emerging Infectious Diseases, University of Texas, San Antonio, Texas 78249"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_3_2","first-page":"105","volume":"58","year":"2009","unstructured":"Centers for Disease Control and Prevention. 2009. 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