{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:33:59Z","timestamp":1791304439682,"version":"4.3.1"},"reference-count":68,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2012,11,1]],"date-time":"2012-11-01T00:00:00Z","timestamp":1351728000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Pathogenic\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Leptospira<\/jats:named-content>\n            spp. are likely to encounter higher concentrations of reactive oxygen species induced by the host innate immune response. In this study, we characterized\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Leptospira interrogans<\/jats:named-content>\n            catalase (KatE), the only annotated catalase found within pathogenic\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Leptospira<\/jats:named-content>\n            species, by assessing its role in resistance to H\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>2<\/jats:sub>\n            -induced oxidative stress and during infection in hamsters. Pathogenic\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">L. interrogans<\/jats:named-content>\n            bacteria had a 50-fold-higher survival rate under H\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>2<\/jats:sub>\n            -induced oxidative stress than did saprophytic\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">L. biflexa<\/jats:named-content>\n            bacteria, and this was predominantly catalase dependent. We also characterized KatE, the only annotated catalase found within pathogenic\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Leptospira<\/jats:named-content>\n            species. Catalase assays performed with recombinant KatE confirmed specific catalase activity, while protein fractionation experiments localized KatE to the bacterial periplasmic space. The insertional inactivation of\n            <jats:italic>katE<\/jats:italic>\n            in pathogenic\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Leptospira<\/jats:named-content>\n            bacteria drastically diminished leptospiral viability in the presence of extracellular H\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>2<\/jats:sub>\n            and reduced virulence in an acute-infection model. Combined, these results suggest that\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">L. interrogans<\/jats:named-content>\n            KatE confers\n            <jats:italic>in vivo<\/jats:italic>\n            resistance to reactive oxygen species induced by the host innate immune response.\n          <\/jats:p>","DOI":"10.1128\/iai.00466-12","type":"journal-article","created":{"date-parts":[[2012,8,28]],"date-time":"2012-08-28T02:57:31Z","timestamp":1346122651000},"page":"3892-3899","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":103,"title":["Leptospira interrogans Catalase Is Required for Resistance to H\n            <sub>2<\/sub>\n            O\n            <sub>2<\/sub>\n            and for Virulence"],"prefix":"10.1128","volume":"80","author":[{"given":"Azad","family":"Eshghi","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristel","family":"Lourdault","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit\u00e9 de Biologie des Spiroch\u00e9tes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald L.","family":"Murray","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Monash University, Clayton, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thanatchaporn","family":"Bartpho","sequence":"additional","affiliation":[{"name":"Melioidosis Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasana W.","family":"Sermswan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Khon Kaen University, Khon Kaen, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Picardeau","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit\u00e9 de Biologie des Spiroch\u00e9tes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Adler","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Monash University, Clayton, Victoria, Australia"},{"name":"Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, Clayton, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brendan","family":"Snarr","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard L.","family":"Zuerner","sequence":"additional","affiliation":[{"name":"Bacterial Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa, USA"},{"name":"Swedish University of Agricultural Sciences, Department of Biomedical Sciences and Veterinary Public Health, Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline E.","family":"Cameron","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.2001.2642"},{"key":"e_1_3_3_3_2","unstructured":"BabudieriB. 1961. 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