{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T07:52:55Z","timestamp":1793087575953,"version":"4.5.0"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2008,9,1]],"date-time":"2008-09-01T00:00:00Z","timestamp":1220227200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Alteration of surface lipoprotein profiles is a key strategy that the Lyme disease pathogen,\n            <jats:italic>Borrelia burgdorferi<\/jats:italic>\n            , has evolved to be maintained within its enzootic cycle between arthropods and mammals. Accumulated evidence indicates that the central regulatory pathway controlling differential gene expression by\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            is the RpoN-RpoS pathway (the \u03c3\n            <jats:sup>54<\/jats:sup>\n            -\u03c3\n            <jats:sup>S<\/jats:sup>\n            sigma factor cascade). It was previously shown that activation of the RpoN-RpoS pathway is controlled by Rrp2, a two-component response regulator and \u03c3\n            <jats:sup>54<\/jats:sup>\n            -dependent transcriptional activator. The role of Rrp2 in the infectious cycle of\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            has not been determined heretofore. In this report, we demonstrate that an\n            <jats:italic>rrp2<\/jats:italic>\n            mutant defective in activating \u03c3\n            <jats:sup>54<\/jats:sup>\n            -dependent transcription was unable to establish infection in mice, but the\n            <jats:italic>rrp2<\/jats:italic>\n            mutant was capable of surviving within ticks during and after tick feeding. Because the\n            <jats:italic>rrp2<\/jats:italic>\n            mutant was defective in the production of OspC, an outer surface lipoprotein essential for mammalian host infection, we further examined whether the loss of infectivity of the\n            <jats:italic>rrp2<\/jats:italic>\n            mutant was solely due to the inability to produce OspC. While transformation with a shuttle vector carrying\n            <jats:italic>ospC<\/jats:italic>\n            under the control of a constitutive\n            <jats:italic>flaB<\/jats:italic>\n            promoter restored infection to an\n            <jats:italic>ospC<\/jats:italic>\n            mutant in immunodeficient SCID mice, it could not rescue the avirulent phenotype of the\n            <jats:italic>rrp2<\/jats:italic>\n            mutant. These data indicate that, in addition to controlling OspC, Rrp2 controls another factor(s) essential for\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            to establish infection in mammals. Furthermore, microarray analyses revealed that 125 and 19 genes were positively and negatively regulated, respectively, by Rrp2, which provides a foundation for future identification of additional Rrp2-dependent virulence determinants in\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/iai.00467-08","type":"journal-article","created":{"date-parts":[[2008,6,24]],"date-time":"2008-06-24T00:55:18Z","timestamp":1214268918000},"page":"3844-3853","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":106,"title":["Essential Role of the Response Regulator Rrp2 in the Infectious Cycle of\n            <i>Borrelia burgdorferi<\/i>"],"prefix":"10.1128","volume":"76","author":[{"given":"Bethany K.","family":"Boardman","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana 46202"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"He","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana 46202"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiming","family":"Ouyang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Texas Southwestern Medical Center, Dallas, Texas 75390"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haijun","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana 46202"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiujuan","family":"Pang","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X. Frank","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana 46202"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-82"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.6.3371-3383.2003"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.15.4129-4136.2000"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7043737"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05813.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.22.7845-7852.2005"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.11.6433-6445.2004"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05860.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01698.x"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05224.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04759.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.21.7390-7402.2004"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2006.11.003"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.1.435-441.2006"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1231043100"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0408536102"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.22.6723-6727.2003"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/37551"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/011676-0"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05593.x"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00037-07"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0306845101"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.8.4759-4764.2000"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00722-9"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.231442498"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.12.7147-7154.2004"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.1.446-455.2001"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2007.05716.x"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01118-07"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01760-06"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(06)22022-6"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(02)58088-5"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.4.1689-1705.2003"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1086\/523764"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200419894"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.25.13865"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03452.x"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.032667699"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0502565102"},{"key":"e_1_3_2_41_2","first-page":"253","year":"1995","unstructured":"Samuels, D. 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