{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T16:25:04Z","timestamp":1793118304495,"version":"4.5.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2020,9,18]],"date-time":"2020-09-18T00:00:00Z","timestamp":1600387200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20180297"],"award-info":[{"award-number":["BK20180297"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31800161"],"award-info":[{"award-number":["31800161"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31770193"],"award-info":[{"award-number":["31770193"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31700157"],"award-info":[{"award-number":["31700157"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2020,9,18]]},"abstract":"<jats:p><jats:named-content content-type=\"genus-species\">Mycoplasma hyopneumoniae<\/jats:named-content>is an important respiratory pathogen of pigs that causes persistent and secondary infections. However, the mechanisms by which this occurs are unclear. In this study, we established air-liquid interface culture systems for pig bronchial epithelial cells (ALI-PBECs) that were comparable to the conditions in the native bronchus<jats:italic>in vivo<\/jats:italic>. We used this ALI-PBECs model to study the infection and migration characteristics of<jats:named-content content-type=\"genus-species\">M. hyopneumoniae<\/jats:named-content><jats:italic>in vitro<\/jats:italic>.<\/jats:p>","DOI":"10.1128\/iai.00470-20","type":"journal-article","created":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T13:38:54Z","timestamp":1596548334000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Paracellular Pathway-Mediated Mycoplasma hyopneumoniae Migration across Porcine Airway Epithelial Barrier under Air-Liquid Interface Conditions"],"prefix":"10.1128","volume":"88","author":[{"given":"Haiyan","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenzhen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Xie","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beibei","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanna","family":"Wei","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Gan","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Yuan","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Ni","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiyan","family":"Xiong","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoqing","family":"Shao","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhixin","family":"Feng","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2013.04.019"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2012.01.003"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12677"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01527-05"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.105475"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/BF03547222"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2006.10.015"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.08.016"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.62.12.5312-5318.1994"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2006.11.003"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12860-018-0162-3"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1513\/AnnalsATS.201407-304AW"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3045"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2008.07.012"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01567-07"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep26748"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12810"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/febs.14582"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0378-1135(99)00176-5"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells5040040"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.4161\/tisb.24997"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a029314"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01868429"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-36054-3"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00054"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01036-13"},{"key":"e_1_3_2_28_2","doi-asserted-by":"crossref","first-page":"1705","DOI":"10.2460\/ajvr.1992.53.09.1705","article-title":"Histochemical and morphologic changes of porcine airway epithelial cells in response to infection with Mycoplasma hyopneumoniae","volume":"53","author":"DeBey MC","year":"1992","unstructured":"DeBey MC, Jacobson CD, Ross RF. 1992. Histochemical and morphologic changes of porcine airway epithelial cells in response to infection with Mycoplasma hyopneumoniae. Am J Vet Res 53:1705\u20131710.","journal-title":"Am J Vet Res"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.62.5.1616-1622.1994"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00974"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2016.01.015"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI15217"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.03.00098803"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2016.09.022"},{"key":"e_1_3_2_35_2","article-title":"M. hyopneumoniae, p 205","author":"Wu Y","year":"2000","unstructured":"Wu Y, Ye Y. 2000. M. hyopneumoniae, p 205. Mycoplasmaology, 2th ed (in Chinese). People\u2019s Medical Publishing House.","journal-title":"Mycoplasmaology"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1089\/vim.2017.0026"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12807"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.62.10.4367-4373.1994"},{"key":"e_1_3_2_39_2","first-page":"537","volume-title":"Diseases of swine","author":"Ross RF","year":"1992","unstructured":"Ross RF. 1992. Mycoplasmal diseases, p 537\u2013551. In Diseases of swine, 7th ed. Iowa State University Press, Ames, IA.","edition":"7"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200801-136OC"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0910061"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.11.7107-7112.2005"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1014719"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.7.002494"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2014.01.011"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64375-1"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2014.07.011"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.16.5.959-961.1982"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2010.09.001"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-018-0541-y"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00470-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00470-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,11]],"date-time":"2024-08-11T08:55:36Z","timestamp":1723366536000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00470-20"}},"subtitle":[],"editor":[{"given":"Guy H.","family":"Palmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,9,18]]},"references-count":49,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2020,9,18]]}},"alternative-id":["10.1128\/IAI.00470-20"],"URL":"https:\/\/doi.org\/10.1128\/iai.00470-20","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,18]]},"assertion":[{"value":"2020-07-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00470-20"}}