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OmpL1 is an outer membrane protein of\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Leptospira<\/jats:named-content>\n                    spp. that is expressed during infection. In this work, we investigated novel features of this protein. We describe that OmpL1 is a novel leptospiral extracellular matrix (ECM)-binding protein and a plasminogen (PLG) receptor. The recombinant protein was expressed in\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Escherichia coli<\/jats:named-content>\n                    BL21(DE3) Star\/pLysS as inclusion bodies, refolded, and purified by metal-chelating chromatography. The protein presented a typical \u03b2-strand secondary structure, as evaluated by circular dichroism spectroscopy. The recombinant protein reacted with antibodies in serum samples from convalescent leptospirosis patients with a high specificity compared to serum samples from individuals with unrelated diseases. These data strengthen the usefulness of OmpL1 as a diagnostic marker of leptospirosis. The characterization of the immunogenicity of recombinant OmpL1 in inoculated BALB\/c mice showed that the protein has the capacity to elicit humoral and cellular immune responses, as denoted by high antibody titers and the proliferation of lymphocytes. We demonstrate that OmpL1 has the ability to mediate attachment to laminin and plasma fibronectin, with\n                    <jats:italic>\n                      K\n                      <jats:sub>D<\/jats:sub>\n                    <\/jats:italic>\n                    (equilibrium dissociation constant) values of 2,099.93 \u00b1 871.03 nM and 1,239.23 \u00b1 506.85 nM, respectively. OmpL1 is also a PLG receptor, with a\n                    <jats:italic>\n                      K\n                      <jats:sub>D<\/jats:sub>\n                    <\/jats:italic>\n                    of 368.63 \u00b1 121.23 nM, capable of generating enzymatically active plasmin. This is the first report that shows and characterizes OmpL1 as an ECM-interacting and a PLG-binding protein of\n                    <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Leptospira<\/jats:named-content>\n                    spp. that may play a role in bacterial pathogenesis when expressed during infection.\n                  <\/jats:p>","DOI":"10.1128\/iai.00474-12","type":"journal-article","created":{"date-parts":[[2012,7,17]],"date-time":"2012-07-17T11:52:16Z","timestamp":1342525936000},"page":"3679-3692","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":76,"title":["OmpL1 Is an Extracellular Matrix- and Plasminogen-Interacting Protein of Leptospira spp"],"prefix":"10.1128","volume":"80","author":[{"given":"Luis G. V.","family":"Fernandes","sequence":"first","affiliation":[{"name":"Centro de Biotecnologia, Instituto Butantan, Avenida Vital Brazil, S\u00e3o Paulo, Brazil"},{"name":"Interunidades em Biotecnologia, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica L.","family":"Vieira","sequence":"additional","affiliation":[{"name":"Centro de Biotecnologia, Instituto Butantan, Avenida Vital Brazil, S\u00e3o Paulo, Brazil"},{"name":"Interunidades em Biotecnologia, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Kirchgatter","sequence":"additional","affiliation":[{"name":"N\u00facleo de Estudos em Mal\u00e1ria, Superintend\u00eancia de Controle de Endemias (SUCEN)\/Instituto de Medicina Tropical de S\u00e3o Paulo, Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivy J.","family":"Alves","sequence":"additional","affiliation":[{"name":"Centro de Biotecnologia, Instituto Butantan, Avenida Vital Brazil, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zenaide M.","family":"de Morais","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Zoonoses Bacterianas do VPS, Faculdade de Medicina Veterin\u00e1ria e Zootecnia da Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvio A.","family":"Vasconcellos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Zoonoses Bacterianas do VPS, Faculdade de Medicina Veterin\u00e1ria e Zootecnia da Universidade de S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliete C.","family":"Romero","sequence":"additional","affiliation":[{"name":"Divis\u00e3o de Biologia Medica, Instituto Adolfo Lutz, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana L. 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