{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T01:46:43Z","timestamp":1791942403580,"version":"4.3.4"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2007,8,1]],"date-time":"2007-08-01T00:00:00Z","timestamp":1185926400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The genes coding for the five outer membrane proteins (OMPs) of the Omp25\/Omp31 family expected to be located in the outer membrane (OM) of rough virulent\n                    <jats:italic>Brucella ovis<\/jats:italic>\n                    PA were inactivated to evaluate their role in virulence and OM properties. The OM properties of the mutant strains and of the mutants complemented with the corresponding wild-type genes were analyzed, in comparison with the parental strain and rough\n                    <jats:italic>B. abortus<\/jats:italic>\n                    RB51, in several tests: (i) binding of anti-Omp25 and anti-Omp31 monoclonal antibodies, (ii) autoagglutination of bacterial suspensions, and (iii) assessment of susceptibility to polymyxin B, sodium deoxycholate, hydrogen peroxide, and nonimmune ram serum. A tight balance of the members of the Omp25\/Omp31 family was seen to be essential for the stability of the\n                    <jats:italic>B. ovis<\/jats:italic>\n                    OM, and important differences between the OMs of\n                    <jats:italic>B. ovis<\/jats:italic>\n                    PA and\n                    <jats:italic>B. abortus<\/jats:italic>\n                    RB51 rough strains were observed. Regarding virulence, the absence of Omp25d and Omp22 from the OM of\n                    <jats:italic>B. ovis<\/jats:italic>\n                    PA led to a drastic reduction in spleen colonization in mice. While the greater susceptibility of the \u0394\n                    <jats:italic>omp22<\/jats:italic>\n                    mutant to nonimmune serum and its difficulty in surviving in the stationary phase might be on the basis of its dramatic attenuation, no defects in the OM able to explain the attenuation of the \u0394\n                    <jats:italic>omp25d<\/jats:italic>\n                    mutant were found, especially considering that the fully virulent \u0394\n                    <jats:italic>omp25c<\/jats:italic>\n                    mutant displayed more important OM defects. Accordingly, Omp25d, and perhaps Omp22, could be directly involved in the penetration and\/or survival of\n                    <jats:italic>B. ovis<\/jats:italic>\n                    inside host cells. This aspect, together with the role of Omp25d and Omp22 in the virulence both of\n                    <jats:italic>B. ovis<\/jats:italic>\n                    in rams and of other\n                    <jats:italic>Brucella<\/jats:italic>\n                    species, should be thoroughly evaluated in future studies.\n                  <\/jats:p>","DOI":"10.1128\/iai.00486-07","type":"journal-article","created":{"date-parts":[[2007,6,11]],"date-time":"2007-06-11T22:32:43Z","timestamp":1181601163000},"page":"4050-4061","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":69,"title":["Role of the Omp25\/Omp31 Family in Outer Membrane Properties and Virulence of\n                    <i>Brucella ovis<\/i>"],"prefix":"10.1128","volume":"75","author":[{"given":"Paola","family":"Caro-Herna\u0301ndez","sequence":"first","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Gene\u0301tica, Edificio Departamental, Universidad de Salamanca, Plaza Doctores de la Reina s\/n, 37007 Salamanca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Ferna\u0301ndez-Lago","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Gene\u0301tica, Edificio Departamental, Universidad de Salamanca, Plaza Doctores de la Reina s\/n, 37007 Salamanca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari\u0301a-Jesu\u0301s","family":"de Miguel","sequence":"additional","affiliation":[{"name":"Centro de Investigacio\u0301n y Tecnologi\u0301a Agroalimentaria del Gobierno de Arago\u0301n, Unidad de Sanidad Animal, Carretera de Montan\u0303ana 930, 50059 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana I.","family":"Marti\u0301n-Marti\u0301n","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologi\u0301a y Gene\u0301tica, Edificio Departamental, Universidad de Salamanca, Plaza Doctores de la Reina s\/n, 37007 Salamanca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Axel","family":"Cloeckaert","sequence":"additional","affiliation":[{"name":"INRA, UR1282, Infectiologie Animale et Sante\u0301 Publique, IASP, Nouzilly F-37380, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari\u0301a-Jesu\u0301s","family":"Grillo\u0301","sequence":"additional","affiliation":[{"name":"Centro de Investigacio\u0301n y Tecnologi\u0301a Agroalimentaria del Gobierno de Arago\u0301n, Unidad de Sanidad Animal, Carretera de Montan\u0303ana 930, 50059 Zaragoza, Spain"},{"name":"Instituto de Agrobiotecnologi\u0301a y Recursos Naturales, CSIC-UPNA, Ctra. 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