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Arguably the most important of these is the two-component, quorum-sensing system\n            <jats:italic>agr<\/jats:italic>\n            . Over the last decade, the controversial presence of a second quorum-sensing system (the TRAP system) has been proposed, and it has been mooted to function as the master regulator of virulence in\n            <jats:italic>S. aureus<\/jats:italic>\n            by modulating\n            <jats:italic>agr<\/jats:italic>\n            . Mutants defective in TRAP are reported to be devoid of\n            <jats:italic>agr<\/jats:italic>\n            expression, lacking in hemolytic activity, essentially deficient in the secretion of virulence determinants, and avirulent in infection models. A number of research groups have questioned the validity of the TRAP findings in recent years; however, a thorough and independent analysis of its role in\n            <jats:italic>S. aureus<\/jats:italic>\n            physiology and pathogenesis has not been forthcoming. Therefore, we have undertaken such an analysis of the TRAP locus of\n            <jats:italic>S. aureus<\/jats:italic>\n            . We found that a\n            <jats:italic>traP<\/jats:italic>\n            mutant was equally hemolytic as the wild-type strain. Furthermore, transcriptional profiling found no alterations in the\n            <jats:italic>traP<\/jats:italic>\n            mutant in expression levels of\n            <jats:italic>agr<\/jats:italic>\n            or in expression levels of multiple\n            <jats:italic>agr<\/jats:italic>\n            -regulated genes (\n            <jats:italic>hla<\/jats:italic>\n            ,\n            <jats:italic>sspA<\/jats:italic>\n            , and\n            <jats:italic>spa<\/jats:italic>\n            ). Analysis of secreted and surface proteins of the\n            <jats:italic>traP<\/jats:italic>\n            mutant revealed no deviation in comparison to the parent. Finally, analysis conducted using a murine model of\n            <jats:italic>S. aureus<\/jats:italic>\n            septic arthritis revealed that, in contrast to an\n            <jats:italic>agr<\/jats:italic>\n            mutant, the\n            <jats:italic>traP<\/jats:italic>\n            mutant was just as virulent as the wild-type strain.\n          <\/jats:p>","DOI":"10.1128\/iai.00491-07","type":"journal-article","created":{"date-parts":[[2007,6,5]],"date-time":"2007-06-05T00:41:41Z","timestamp":1181004101000},"page":"4519-4527","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Inactivation of\n            <i>traP<\/i>\n            Has No Effect on the Agr Quorum-Sensing System or Virulence of\n            <i>Staphylococcus aureus<\/i>"],"prefix":"10.1128","volume":"75","author":[{"given":"Lindsey N.","family":"Shaw","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathobiology and Bond Life Sciences Center, University of Missouri, Columbia, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ing-Marie","family":"Jonsson","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Inflammation Research, University of Goteborg, Goteborg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vineet K.","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, A.T. Still University of Health Sciences, Kirksville College of Osteopathic Medicine, Kirksville, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrej","family":"Tarkowski","sequence":"additional","affiliation":[{"name":"Department of Rheumatology and Inflammation Research, University of Goteborg, Goteborg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George C.","family":"Stewart","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology and Bond Life Sciences Center, University of Missouri, Columbia, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.61.9.3879-3885.1993"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00580-06"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00112"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01097-06"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0196-9781(00)00272-2"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/345879"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M005446200"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5362.438"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.5.1619"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5452.391a"},{"key":"e_1_3_2_12_2","first-page":"48","volume":"437","year":"2005","unstructured":"Balaban, N., P. 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