{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T12:16:30Z","timestamp":1768565790479,"version":"3.49.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2018,3,1]],"date-time":"2018-03-01T00:00:00Z","timestamp":1519862400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"PARAVAC, European Commission Grant","award":["265862\/FP-7-KBBE"],"award-info":[{"award-number":["265862\/FP-7-KBBE"]}]},{"name":"PARAVAC, European Commission Grant","award":["265862\/FP-7-KBBE"],"award-info":[{"award-number":["265862\/FP-7-KBBE"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Bacillus subtilis<\/jats:named-content>\n            is known as an endospore- and biofilm-forming bacterium with probiotic properties. We have recently developed a method for displaying heterologous proteins on the surface of\n            <jats:named-content content-type=\"genus-species\">B. subtilis<\/jats:named-content>\n            biofilms by introducing the coding sequences of the protein of interest into the bacterial genome to generate a fusion protein linked to the C terminus of the biofilm matrix protein TasA. Although\n            <jats:named-content content-type=\"genus-species\">B. subtilis<\/jats:named-content>\n            is a regular component of the gut microflora, we constructed a series of recombinant\n            <jats:named-content content-type=\"genus-species\">B. subtilis<\/jats:named-content>\n            strains that were tested for their ability to be used to immunize dogs following oral application of the spores. Specifically, we tested recombinant spores of\n            <jats:named-content content-type=\"genus-species\">B. subtilis<\/jats:named-content>\n            carrying either the fluorescent protein mCherry or else selected antigenic peptides (tropomyosin and paramyosin) from\n            <jats:named-content content-type=\"genus-species\">Echinococcus granulosus<\/jats:named-content>\n            , a zoonotic intestinal tapeworm of dogs and other carnivores. The application of the recombinant\n            <jats:named-content content-type=\"genus-species\">B. subtilis<\/jats:named-content>\n            spores led to the colonization of the gut with recombinant\n            <jats:named-content content-type=\"genus-species\">B. subtilis<\/jats:named-content>\n            but did not cause any adverse effect on the health of the animals. As measured by enzyme-linked immunosorbent assay and immunoblotting, the dogs were able to develop a humoral immune response against mCherry as well as against\n            <jats:named-content content-type=\"genus-species\">E. granulosus<\/jats:named-content>\n            antigenic peptides. Interestingly, the sera of dogs obtained after immunization with recombinant spores of\n            <jats:named-content content-type=\"genus-species\">E. granulosus<\/jats:named-content>\n            peptides were able to recognize\n            <jats:named-content content-type=\"genus-species\">E. granulosus<\/jats:named-content>\n            protoscoleces, which represent the infective form of the head of the tapeworms. These results represent an essential step toward the establishment of\n            <jats:named-content content-type=\"genus-species\">B. subtilis<\/jats:named-content>\n            as an enteric vaccine agent.\n          <\/jats:p>","DOI":"10.1128\/iai.00495-17","type":"journal-article","created":{"date-parts":[[2017,12,11]],"date-time":"2017-12-11T16:15:36Z","timestamp":1513008936000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Oral Application of Recombinant Bacillus subtilis Spores to Dogs Results in a Humoral Response against Specific Echinococcus granulosus Paramyosin and Tropomyosin Antigens"],"prefix":"10.1128","volume":"86","author":[{"given":"C\u00e9dric M.","family":"Vogt","sequence":"first","affiliation":[{"name":"Institute of Virology, Vetsuisse, University of Zurich, Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Teresa","family":"Arm\u00faa-Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Vetsuisse, University of Zurich, Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kurt","family":"Tobler","sequence":"additional","affiliation":[{"name":"Institute of Virology, Vetsuisse, University of Zurich, Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Hilbe","sequence":"additional","affiliation":[{"name":"Laboratory for Animal Model Pathology, Institute of Pathology, Vetsuisse, University of Zurich, Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudio","family":"Aguilar","sequence":"additional","affiliation":[{"name":"rqmicro Ltd., ETH, Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathias","family":"Ackermann","sequence":"additional","affiliation":[{"name":"Institute of Virology, Vetsuisse, University of Zurich, Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Deplazes","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Vetsuisse, University of Zurich, Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Eichwald","sequence":"additional","affiliation":[{"name":"Institute of Virology, Vetsuisse, University of Zurich, Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.10.020"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2014.04.050"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-014-0077-8"},{"key":"e_1_3_3_5_2","volume-title":"Bronchi-Shield Oral-First oral Bordetella bronchiseptica vaccine approved for use in dogs","author":"Chesnut M","year":"2012","unstructured":"Chesnut M. 2012. 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