{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T13:05:45Z","timestamp":1792674345434,"version":"4.5.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We have previously shown that during late stages of the infectious process, serogroup B meningococci (MenB) are able to escape the phagosome of\n            <jats:italic>in vitro<\/jats:italic>\n            -infected human epithelial cells. They then multiply in the cytosolic environment and spread intracellularly and to surrounding cells by exploiting the microtubule cytoskeleton, as suggested by results of infections in the presence of microtubule inhibitors and evidence of nanotubes connecting neighboring cells. In this study, by using microtubule binding assays with purified microtubule asters and bundles and microtubule bundles synthesized\n            <jats:italic>in vitro<\/jats:italic>\n            , we demonstrate that the MenB capsule directly mediates the interaction between bacteria and microtubules. The direct interaction between the microtubules and the MenB capsular polysaccharide was confirmed by coimmunoprecipitation experiments. Unexpectedly, serogroup C meningococci (MenC), which have a capsular polysaccharide that differs from that of MenB only by its anomeric linkage, \u03b1(2\u21929) instead of \u03b1(2\u21928), were not able to interact with the microtubules, and the lack of interaction was not due to capsular polysaccharide\n            <jats:italic>O<\/jats:italic>\n            -acetylation that takes place in most MenC strains but not in MenB strains. Moreover, we demonstrate that the MenB capsular polysaccharide inhibits tubulin polymerization\n            <jats:italic>in vitro<\/jats:italic>\n            . Thus, at variance with MenC, MenB may interfere with microtubule dynamics during cell infection.\n          <\/jats:p>","DOI":"10.1128\/iai.00501-13","type":"journal-article","created":{"date-parts":[[2013,10,29]],"date-time":"2013-10-29T03:20:30Z","timestamp":1383016830000},"page":"265-274","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Serogroup-Specific Interaction of Neisseria meningitidis Capsular Polysaccharide with Host Cell Microtubules and Effects on Tubulin Polymerization"],"prefix":"10.1128","volume":"82","author":[{"given":"Adelfia","family":"Tal\u00e0","sequence":"first","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biologiche e Ambientali, Universit\u00e0 del Salento, Lecce, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Cogli","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biologiche e Ambientali, Universit\u00e0 del Salento, Lecce, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"De Stefano","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Ambientali, Seconda Universit\u00e0 di Napoli, Caserta, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcella","family":"Cammarota","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Sperimentale, Seconda Universit\u00e0 di Napoli, Naples, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Rita","family":"Spinosa","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biologiche e Ambientali, Universit\u00e0 del Salento, Lecce, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Bucci","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biologiche e Ambientali, Universit\u00e0 del Salento, Lecce, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pietro","family":"Alifano","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biologiche e Ambientali, Universit\u00e0 del Salento, Lecce, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(02)02402-2"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.04.063"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/649209"},{"key":"e_1_3_3_5_2","first-page":"521","article-title":"Meningococcal vaccines: WHO position paper, November 2011","volume":"86","author":"World Health Organization","year":"2011","unstructured":"World Health Organization. 2011. 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