{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T02:25:19Z","timestamp":1784082319514,"version":"3.55.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The major predisposing factor for melioidosis is diabetes mellitus, but no immunological mechanisms have been investigated to explain this. In this study, polymorphonuclear neutrophil (PMN) responses to\n            <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n            , the causative agent of melioidosis, in healthy and diabetic Thai subjects were determined by flow cytometry. The results showed that\n            <jats:italic>B. pseudomallei<\/jats:italic>\n            displayed reduced uptake by PMNs compared to\n            <jats:italic>Salmonella enterica<\/jats:italic>\n            serovar Typhimurium and\n            <jats:italic>Escherichia coli<\/jats:italic>\n            . Additionally, intracellular survival of\n            <jats:italic>B. pseudomallei<\/jats:italic>\n            was detected throughout a 24-h period, indicating the intrinsic resistance of\n            <jats:italic>B. pseudomallei<\/jats:italic>\n            to killing by PMNs. Moreover, PMNs from diabetic subjects displayed impaired phagocytosis of\n            <jats:italic>B. pseudomallei<\/jats:italic>\n            , reduced migration in response to interleukin-8, and an inability to delay apoptosis. These data show that\n            <jats:italic>B. pseudomallei<\/jats:italic>\n            is intrinsically resistant to phagocytosis and killing by PMNs. These observations, together with the impaired migration and apoptosis in diabetes mellitus, may explain host susceptibility in melioidosis.\n          <\/jats:p>","DOI":"10.1128\/iai.00503-08","type":"journal-article","created":{"date-parts":[[2008,10,28]],"date-time":"2008-10-28T00:15:07Z","timestamp":1225152907000},"page":"456-463","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":113,"title":["Human Polymorphonuclear Neutrophil Responses to\n            <i>Burkholderia pseudomallei<\/i>\n            in Healthy and Diabetic Subjects"],"prefix":"10.1128","volume":"77","author":[{"given":"Sujin","family":"Chanchamroen","sequence":"first","affiliation":[{"name":"The Center for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen 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