{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:02:06Z","timestamp":1791266526776,"version":"4.1.0"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2015,10,1]],"date-time":"2015-10-01T00:00:00Z","timestamp":1443657600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The Gram-positive bacterium<jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>is a facultative intracellular pathogen that relies on the regulated secretion and activity of a variety of proteins that sustain life within diverse environments. PrsA2 has recently been identified as a secreted peptidyl-prolyl<jats:italic>cis<\/jats:italic>\/<jats:italic>trans<\/jats:italic>isomerase and chaperone that is dispensable for bacterial growth in broth culture but essential for<jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>virulence. Following host infection, PrsA2 contributes to the proper folding and activity of secreted proteins that are required for bacterial replication within the host cytosol and for bacterial spread to adjacent cells. PrsA2 is one member of a family of Gram-positive secretion chaperones that appear to play important roles in bacterial physiology; however, it is not known how these proteins recognize their substrate proteins or the degree to which their function is conserved across diverse Gram-positive species. We therefore examined PrsA proteins encoded by a variety of Gram-positive bacteria for functional complementation of<jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>mutants lacking<jats:italic>prsA2<\/jats:italic>. PrsA homologues encoded by<jats:named-content content-type=\"genus-species\">Bacillus subtilis<\/jats:named-content>,<jats:named-content content-type=\"genus-species\">Streptococcus pyogenes<\/jats:named-content>,<jats:named-content content-type=\"genus-species\">Streptococcus pneumoniae<\/jats:named-content>,<jats:named-content content-type=\"genus-species\">Streptococcus mutans<\/jats:named-content>,<jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>, and<jats:named-content content-type=\"genus-species\">Lactococcus lactis<\/jats:named-content>were examined for functional complementation of a variety of<jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>PrsA2-associated phenotypes central to<jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>pathogenesis and bacterial cell physiology. Our results indicate that while selected aspects of PrsA2 function are broadly conserved among diverse Gram-positive bacteria, PrsA2 exhibits unique specificity for<jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>target proteins required for pathogenesis. The<jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>PrsA2 chaperone thus appears evolutionarily optimized for virulence factor secretion within the host cell cytosol while still maintaining aspects of activity relevant to more general features of Gram-positive protein translocation.<\/jats:p>","DOI":"10.1128\/iai.00504-15","type":"journal-article","created":{"date-parts":[[2015,7,28]],"date-time":"2015-07-28T01:01:53Z","timestamp":1438045313000},"page":"4028-4041","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Identification of Conserved and Species-Specific Functions of the Listeria monocytogenes PrsA2 Secretion Chaperone"],"prefix":"10.1128","volume":"83","author":[{"given":"Laty A.","family":"Cahoon","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy E.","family":"Freitag","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2013.03.016"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02278.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/10408410490499872"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1861"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.12.57"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268805005376"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0505.990502"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmwh.2008.01.005"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2008.00404.x"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01366-06"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.05.011"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00532-10"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03534.x"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.4.1917-1926.2005"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00845-07"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2011.01.005"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394590-7.00007-5"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.4.1293-1298.1992"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.21.8336"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb02158.x"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2171"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14029"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.2.1323-1338.2006"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2014.00013"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2012.08040.x"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01640.x"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb01901.x"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.06264-11"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.8.3932-3942.2002"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.026765-0"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01059-06"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/omi.12014"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00280-09"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00016-09"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.05307-11"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2011.07665.x"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01616.x"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M003804200"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.25511-0"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M400861200"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07188.x"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03413.x"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00314-08"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.11.3770-3778.1990"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6807-9-17"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01247.x"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M510014200"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033516"},{"key":"e_1_3_3_50_2","first-page":"2171","article-title":"Temperature-dependent expression of flagella of Listeria monocytogenes studied by electron microscopy, SDS-PAGE and western blotting","volume":"134","author":"Peel M","year":"1988","unstructured":"Peel M, Donachie W, Shaw A. 1988. Temperature-dependent expression of flagella of Listeria monocytogenes studied by electron microscopy, SDS-PAGE and western blotting. J Gen Microbiol 134:2171\u20132178.","journal-title":"J Gen Microbiol"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2008.05.006"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.11.3629-3636.1989"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.21.6295-6307.2003"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.4.1459"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01001-06"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2016"},{"key":"e_1_3_3_57_2","doi-asserted-by":"crossref","first-page":"2005","DOI":"10.4049\/jimmunol.139.6.2005","article-title":"Adoptive transfer of immunity to Listeria monocytogenes. The influence of in vitro stimulation on lymphocyte subset requirements","volume":"139","author":"Bishop DK","year":"1987","unstructured":"Bishop DK, Hinrichs DJ. 1987. Adoptive transfer of immunity to Listeria monocytogenes. The influence of in vitro stimulation on lymphocyte subset requirements J Immunol 139:2005\u20132009.","journal-title":"J Immunol"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt381"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00504-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00504-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,12]],"date-time":"2023-08-12T15:36:37Z","timestamp":1691854597000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00504-15"}},"subtitle":[],"editor":[{"given":"A.","family":"Camilli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,10]]},"references-count":58,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2015,10]]}},"alternative-id":["10.1128\/IAI.00504-15"],"URL":"https:\/\/doi.org\/10.1128\/iai.00504-15","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,10]]},"assertion":[{"value":"2015-04-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-07-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-09-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}