{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T11:27:26Z","timestamp":1784287646905,"version":"3.55.0"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T00:00:00Z","timestamp":1712620800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2024,4,9]],"date-time":"2024-04-09T00:00:00Z","timestamp":1712620800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32161143011"],"award-info":[{"award-number":["32161143011"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31972685"],"award-info":[{"award-number":["31972685"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"MOST | National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31920103015"],"award-info":[{"award-number":["31920103015"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2024,4,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:sec>\n            <jats:title\/>\n            <jats:p>\n              The inflammasome is a pivotal component of the innate immune system, acting as a multiprotein complex that plays an essential role in detecting and responding to microbial infections.\n              <jats:italic>Salmonella<\/jats:italic>\n              Enteritidis have evolved multiple mechanisms to regulate inflammasome activation and evade host immune system clearance. Through screening\n              <jats:italic>S<\/jats:italic>\n              . Enteritidis C50336\u0394\n              <jats:italic>fliC<\/jats:italic>\n              transposon mutant library, we found that the insertion mutant of\n              <jats:italic>dinJ<\/jats:italic>\n              increased inflammasome activation. In this study, we demonstrated the genetic connection between the antitoxin DinJ and the toxin YafQ in\n              <jats:italic>S<\/jats:italic>\n              . Enteritidis, confirming their co-transcription. The deletion mutant \u0394\n              <jats:italic>fliC<\/jats:italic>\n              \u0394\n              <jats:italic>dinJ<\/jats:italic>\n              increased cell death and IL-1\u03b2 secretion in J774A.1 cells. Western blotting analysis further showed elevated cleaved Caspase-1 product (p10 subunits) and IL-1\u03b2 secretion in cells infected with \u0394\n              <jats:italic>fliC<\/jats:italic>\n              \u0394\n              <jats:italic>dinJ<\/jats:italic>\n              compared to cells infected with \u0394\n              <jats:italic>fliC<\/jats:italic>\n              . DinJ was found to inhibit canonical inflammasome activation using primary bone marrow-derived macrophages (BMDMs) from\n              <jats:italic>Casp<\/jats:italic>\n              <jats:sup>-\/-<\/jats:sup>\n              C57BL\/6 mice. Furthermore, DinJ specifically inhibited NLRP3 inflammasome activation, as demonstrated in BMDMs from\n              <jats:italic>Nlrp3<\/jats:italic>\n              <jats:sup>\n                <jats:italic>-\/-<\/jats:italic>\n              <\/jats:sup>\n              and\n              <jats:italic>Nlrc4<\/jats:italic>\n              <jats:sup>\n                <jats:italic>-\/-<\/jats:italic>\n              <\/jats:sup>\n              mice. Fluorescence resonance energy transfer (FRET) experiments confirmed the translocation of DinJ into host cells during infection. Finally, we revealed that DinJ could inhibit the secretion of IL-1\u03b2 and IL-18\n              <jats:italic>in vivo<\/jats:italic>\n              , contributing to\n              <jats:italic>S<\/jats:italic>\n              . Enteritidis evading host immune clearance. In summary, our findings provide insights into the role of DinJ in modulating the inflammasome response during\n              <jats:italic>S<\/jats:italic>\n              . Enteritidis infection, highlighting its impact on inhibiting inflammasome activation and immune evasion.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/iai.00505-23","type":"journal-article","created":{"date-parts":[[2024,3,17]],"date-time":"2024-03-17T17:21:21Z","timestamp":1710696081000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["<i>Salmonella<\/i>\n            Enteritidis antitoxin DinJ inhibits NLRP3-dependent canonical inflammasome activation in macrophages"],"prefix":"10.1128","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6833-5358","authenticated-orcid":false,"given":"Dan","family":"Gu","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis\/Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ang","family":"Li","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis\/Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xirui","family":"Zang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis\/Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Huang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis\/Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaxin","family":"Guo","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis\/Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2214-3358","authenticated-orcid":false,"given":"Xinan","family":"Jiao","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis\/Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7466-872X","authenticated-orcid":false,"given":"Zhiming","family":"Pan","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Zoonosis\/Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Key Laboratory of Prevention and Control of Biological Hazard Factors (Animal Origin) for Agrifood Safety and Quality, Ministry of Agriculture of China, Yangzhou University, Yangzhou, Jiangsu, China"},{"name":"Joint International Research Laboratory of Agriculture and Agri-product Safety of the Ministry of Education, Yangzhou University, Yangzhou, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.03.046"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1169841"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20130627"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10510"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-007-9047-x"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature15514"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003142"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006035"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.108832"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2016.11.009"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-41171-2_3"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006925"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21186-4"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.MA0318-112RR"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009718"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2017.07.011"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1011381"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-021-00661-1"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2011.02618.x"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep28759"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2006.00563.x"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.13641"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.573006"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00935-20"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S378157"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10050911"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2023.127460"},{"key":"e_1_3_4_29_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20100257"},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00008"},{"key":"e_1_3_4_31_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00085"},{"key":"e_1_3_4_32_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1306376110"},{"key":"e_1_3_4_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1344"},{"key":"e_1_3_4_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.02803-14"},{"key":"e_1_3_4_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1346"},{"key":"e_1_3_4_36_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2000382"},{"key":"e_1_3_4_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e14220"},{"key":"e_1_3_4_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep38204"},{"key":"e_1_3_4_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.20681"},{"key":"e_1_3_4_40_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002394"},{"key":"e_1_3_4_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02172-15"},{"key":"e_1_3_4_42_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10565-021-09587-z"},{"key":"e_1_3_4_43_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-020-01919-z"},{"key":"e_1_3_4_44_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2017.1323157"},{"key":"e_1_3_4_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10394"},{"key":"e_1_3_4_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.6.1630-1639.2002"},{"key":"e_1_3_4_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.5.1310-1319.1996"},{"key":"e_1_3_4_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90007-x"},{"key":"e_1_3_4_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.16.5486-5495.2004"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.00505-23","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/iai.00505-23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,15]],"date-time":"2024-05-15T23:17:12Z","timestamp":1715815032000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/iai.00505-23"}},"subtitle":[],"editor":[{"given":"Manuela","family":"Raffatellu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,4,9]]},"references-count":48,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2024,4,9]]}},"alternative-id":["10.1128\/iai.00505-23"],"URL":"https:\/\/doi.org\/10.1128\/iai.00505-23","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,4,9]]},"assertion":[{"value":"2023-12-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-03-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00505-23"}}