{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T18:07:21Z","timestamp":1787854041829,"version":"build-2784847793"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:00:00Z","timestamp":1765843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:00:00Z","timestamp":1765843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2025,12,16]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Placental malaria (PM) causes mortality and severe morbidity in areas with stable\n                      <jats:italic toggle=\"yes\">Plasmodium falciparum<\/jats:italic>\n                      transmission. The selective placental accumulation of\n                      <jats:italic toggle=\"yes\">P. falciparum<\/jats:italic>\n                      -infected erythrocytes (IEs) is mediated by VAR2CSA, a PfEMP1-type parasite ligand that binds exclusively to placenta-restricted CSA. VAR2CSA-specific IgG is therefore generally restricted to women exposed to\n                      <jats:italic toggle=\"yes\">P. falciparum<\/jats:italic>\n                      infection during pregnancy. However, widespread acquisition of VAR2CSA-reactive IgG outside pregnancy among Colombian and Brazilian individuals has been reported, supposedly due to cross-reactivity between VAR2CSA and the\n                      <jats:italic toggle=\"yes\">P. vivax<\/jats:italic>\n                      -specific antigen PvDBP. Here, we measured levels and Fc-afucosylation of VAR2CSA-reactive IgG in plasma from pregnant Brazilian women at delivery, using full-length VAR2CSA (FV2) expressed in baculovirus-transfected insect cells (FV2\n                      <jats:sub>BIC<\/jats:sub>\n                      ) or Chinese hamster ovary cells (FV2\n                      <jats:sub>CHO<\/jats:sub>\n                      ) as well as the corresponding native antigen (IT4VAR04) on the IE surface. We also measured levels of IgG specific for GLURP (\n                      <jats:italic toggle=\"yes\">P. falciparum-<\/jats:italic>\n                      specific) and PvDBP (\n                      <jats:italic toggle=\"yes\">P. vivax<\/jats:italic>\n                      -specific). FV2\n                      <jats:sub>CHO<\/jats:sub>\n                      -specific IgG levels were lower than FV2\n                      <jats:sub>BIC<\/jats:sub>\n                      -reactive IgG levels. Furthermore, only FV2\n                      <jats:sub>CHO<\/jats:sub>\n                      -specific IgG was restricted to women exposed to\n                      <jats:italic toggle=\"yes\">P. falciparum<\/jats:italic>\n                      during pregnancy. Levels of PvDBP-specific IgG were significantly higher among\n                      <jats:italic toggle=\"yes\">P. vivax<\/jats:italic>\n                      -exposed pregnant women but did not correlate with FV2-specific IgG levels. Finally, FV2\n                      <jats:sub>CHO<\/jats:sub>\n                      -specific IgG was markedly Fc-afucosylated in contrast to FV2\n                      <jats:sub>BIC<\/jats:sub>\n                      -reactive IgG. Our findings caution against using levels of IgG reacting with recombinant proteins expressed in insect cells as a measure of exposure to VAR2CSA during pregnancy, at least in South America. Furthermore, our data do not support the hypothesis that exposure to PvDBP induces IgG that cross-reacts with VAR2CSA and contributes to protection against PM.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/iai.00516-25","type":"journal-article","created":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T12:50:34Z","timestamp":1761915034000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["VAR2CSA-reactive IgG in Brazilian women exposed to\n                    <i>Plasmodium falciparum<\/i>\n                    or\n                    <i>P. vivax<\/i>\n                    infection during pregnancy"],"prefix":"10.1128","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5826-6782","authenticated-orcid":true,"given":"Maria In\u00eas","family":"dos Santos","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036rp1748","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Parasitology, Institute of Biomedical Sciences, University of S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]},{"id":[{"id":"https:\/\/ror.org\/035b05819","id-type":"ROR","asserted-by":"publisher"}],"name":"Centre for translational Medicine and Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen","place":["Copenhagen, Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1863-1914","authenticated-orcid":true,"given":"Jamille Greg\u00f3rio","family":"Dombrowski","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036rp1748","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Parasitology, Institute of Biomedical Sciences, University of S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6155-4896","authenticated-orcid":false,"given":"Henriette","family":"Hoffmann-Veltung","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035b05819","id-type":"ROR","asserted-by":"publisher"}],"name":"Centre for translational Medicine and Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen","place":["Copenhagen, Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6190-3324","authenticated-orcid":true,"given":"Maria del Pilar","family":"Quintana","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035b05819","id-type":"ROR","asserted-by":"publisher"}],"name":"Centre for translational Medicine and Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen","place":["Copenhagen, Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1227-3845","authenticated-orcid":true,"given":"Claudio Romero Farias","family":"Marinho","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036rp1748","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Parasitology, Institute of Biomedical Sciences, University of S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1698-4927","authenticated-orcid":true,"given":"Lars","family":"Hviid","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035b05819","id-type":"ROR","asserted-by":"publisher"}],"name":"Centre for translational Medicine and Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen","place":["Copenhagen, Denmark"]},{"id":[{"id":"https:\/\/ror.org\/03mchdq19","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Infectious Diseases, Rigshospitalet","place":["Copenhagen, Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9876-0248","authenticated-orcid":true,"given":"Mary","family":"Lopez-Perez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035b05819","id-type":"ROR","asserted-by":"publisher"}],"name":"Centre for translational Medicine and Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen","place":["Copenhagen, Denmark"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","unstructured":"WHO. 2024. 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