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An acute inflammatory response at the intestine helps to prevent\n            <jats:named-content content-type=\"genus-species\">Salmonella<\/jats:named-content>\n            dissemination to systemic sites. Flagella contribute to this response by providing motility and FliC-mediated signaling through pattern recognition receptors. In a previous work, we reported a high frequency (11 out of 25) of\n            <jats:italic>S<\/jats:italic>\n            . Dublin isolates lacking flagella in a collection obtained from humans and cattle. The aflagellate strains were impaired in their proinflammatory properties\n            <jats:italic>in vitro<\/jats:italic>\n            and\n            <jats:italic>in vivo<\/jats:italic>\n            . The aim of this work was to elucidate the underlying cause of the absence of flagella in\n            <jats:italic>S<\/jats:italic>\n            . Dublin isolates. We report here that class 3 flagellar genes are repressed in the human aflagellate isolates, due to impaired secretion of FliA anti-sigma factor FlgM. This phenotype is due to an in-frame 42-nucleotide deletion in the\n            <jats:italic>fliE<\/jats:italic>\n            gene, which codes for a protein located in the flagellar basal body. The deletion is predicted to produce a protein lacking amino acids 18 to 31. The aflagellate phenotype was highly stable; revertants were obtained only when\n            <jats:italic>fliA<\/jats:italic>\n            was artificially overexpressed combined with several successive passages in motility agar. DNA sequence analysis revealed that motile revertants resulted from duplications of DNA sequences in\n            <jats:italic>fliE<\/jats:italic>\n            adjacent to the deleted region. These duplications produced a FliE protein of similar length to the wild type and demonstrate that amino acids 18 to 31 of FliE are not essential. The same deletion was detected in\n            <jats:italic>S<\/jats:italic>\n            . Dublin isolates obtained from cattle, indicating that this mutation circulates in nature.\n          <\/jats:p>","DOI":"10.1128\/iai.00517-17","type":"journal-article","created":{"date-parts":[[2017,10,24]],"date-time":"2017-10-24T00:30:58Z","timestamp":1508805058000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["A Naturally Occurring Deletion in FliE from Salmonella enterica Serovar Dublin Results in an Aflagellate Phenotype and Defective Proinflammatory Properties"],"prefix":"10.1128","volume":"86","author":[{"given":"Sebasti\u00e1n","family":"Sas\u00edas","sequence":"first","affiliation":[{"name":"Departamento de Desarrollo Biotecnol\u00f3gico, Instituto de Higiene, Facultad de Medicina, Universidad de la Rep\u00fablica, Montevideo, Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana","family":"Mart\u00ednez-Sanguin\u00e9","sequence":"additional","affiliation":[{"name":"Departamento de Desarrollo Biotecnol\u00f3gico, Instituto de Higiene, Facultad de Medicina, Universidad de la Rep\u00fablica, Montevideo, Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Betancor","sequence":"additional","affiliation":[{"name":"Departamento de Bacteriolog\u00eda y Virolog\u00eda, Instituto de Higiene, Facultad de Medicina, Universidad de la Rep\u00fablica, Montevideo, Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arac\u00ed","family":"Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Departamento de Bacteriolog\u00eda y Virolog\u00eda, Instituto de Higiene, Facultad de Medicina, Universidad de la Rep\u00fablica, Montevideo, Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"D'Alessandro","sequence":"additional","affiliation":[{"name":"Departamento de Desarrollo Biotecnol\u00f3gico, Instituto de Higiene, Facultad de Medicina, Universidad de la Rep\u00fablica, Montevideo, Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andr\u00e9s","family":"Iriarte","sequence":"additional","affiliation":[{"name":"Departamento de Desarrollo Biotecnol\u00f3gico, Instituto de Higiene, Facultad de Medicina, Universidad de la Rep\u00fablica, Montevideo, Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 A.","family":"Chabalgoity","sequence":"additional","affiliation":[{"name":"Departamento de Desarrollo Biotecnol\u00f3gico, Instituto de Higiene, Facultad de Medicina, Universidad de la Rep\u00fablica, Montevideo, Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luc\u00eda","family":"Yim","sequence":"additional","affiliation":[{"name":"Departamento de Desarrollo Biotecnol\u00f3gico, Instituto de Higiene, Facultad de Medicina, Universidad de la Rep\u00fablica, Montevideo, Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001921"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm6514a2"},{"key":"e_1_3_3_4_2","volume-title":"XX Congreso Latinoamericano de Microbiologia","author":"Palacio R","year":"2010","unstructured":"Palacio R, Alfonso SAA, Legnani M, Da Silva A, Castro M, Camou T, Lopez D, Strozzi D, Rosa R. 2010. 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