{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T15:33:19Z","timestamp":1758814399202},"reference-count":59,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Linear plasmid lp54 is one of the most highly conserved and differentially expressed elements of the segmented genome of the Lyme disease spirochete\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Borrelia burgdorferi<\/jats:named-content>\n            . We previously reported that deletion of a 4.1-kb region of lp54 (\n            <jats:italic>bba01<\/jats:italic>\n            to\n            <jats:italic>bba07<\/jats:italic>\n            [\n            <jats:italic>bba01-bba07<\/jats:italic>\n            ]) led to a slight attenuation of tick-transmitted infection in mice following challenge with a large number of infected ticks. In the current study, we reduced the number of ticks in the challenge to more closely mimic the natural dose and found a profound defect in tick-transmitted infection of the\n            <jats:italic>bba01-bba07<\/jats:italic>\n            mutant relative to wild-type\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. burgdorferi<\/jats:named-content>\n            . We next focused on deletion of\n            <jats:italic>bba03<\/jats:italic>\n            as the most likely cause of this mutant phenotype, as previous studies have shown that expression of\n            <jats:italic>bba03<\/jats:italic>\n            is increased by culture conditions that simulate tick feeding. Consistent with this hypothesis, we demonstrated increased expression of\n            <jats:italic>bba03<\/jats:italic>\n            by spirochetes in fed relative to unfed ticks. We also observed that a\n            <jats:italic>bba03<\/jats:italic>\n            deletion mutant, although fully competent by itself, did not efficiently infect mice when transmitted by ticks that were simultaneously coinfected with wild-type\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">B. burgdorferi<\/jats:named-content>\n            . These results suggest that BBA03 provides a competitive advantage to spirochetes carrying this protein during tick transmission to a mammalian host in the natural infectious cycle.\n          <\/jats:p>","DOI":"10.1128\/iai.00521-12","type":"journal-article","created":{"date-parts":[[2012,7,31]],"date-time":"2012-07-31T07:45:32Z","timestamp":1343720732000},"page":"3501-3511","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Competitive Advantage of Borrelia burgdorferi with Outer Surface Protein BBA03 during Tick-Mediated Infection of the Mammalian Host"],"prefix":"10.1128","volume":"80","author":[{"given":"Aaron","family":"Bestor","sequence":"first","affiliation":[{"name":"Laboratory of Zoonotic Pathogens, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan O. 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