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Mast cells are important effector cells of tissue inflammation during infection with parasites. Membrane-bridging SNARE (soluble\n            <jats:italic>N<\/jats:italic>\n            -ethylmaleimide-sensitive factor attachment protein receptor) complexes are critical for fusion during exocytosis. Although\n            <jats:named-content content-type=\"genus-species\">T. vaginalis<\/jats:named-content>\n            -derived secretory products (TvSP) have been shown to induce exocytosis in mast cells, information regarding the signaling mechanisms between mast cell activation and TvSP is limited. In this study, we found that SNAP23-dependent surface trafficking of LTB\n            <jats:sub>4<\/jats:sub>\n            receptor 1 (BLT1) is required for nicotinamide adenine dinucleotide phosphate oxidase 2 (NOX2)-mediated exocytotic degranulation of mast cells induced by TvSP. First, stimulation with TvSP induced exocytotic degranulation and reactive oxygen species (ROS) generation in HMC-1 cells. Next, TvSP-induced ROS generation and exocytosis were strongly inhibited by transfection of BLT1 small interfering RNA (siRNA). TvSP induced trafficking of BLT1 from the cytosol to the plasma membrane. We also found that knockdown of SNAP23 abrogated TvSP-induced ROS generation, exocytosis, and surface trafficking of BLT1 in HMC-1 cells. By coimmunoprecipitation, there was a physical interaction between BLT1 and SNAP23 in TvSP-stimulated HMC-1 cells. Taken together, our results suggest that SNAP23-dependent surface trafficking of BLT1 is essential for exocytosis in human mast cells induced by\n            <jats:named-content content-type=\"genus-species\">T. vaginalis<\/jats:named-content>\n            -secreted LTB\n            <jats:sub>4<\/jats:sub>\n            . Our data collectively demonstrate a novel regulatory mechanism for SNAP23-dependent mast cell activation of\n            <jats:named-content content-type=\"genus-species\">T. vaginalis<\/jats:named-content>\n            -secreted LTB\n            <jats:sub>4<\/jats:sub>\n            involving surface trafficking of BLT1. These results can help to explain how the cross talk mechanism between parasite and host can govern deliberately tissue inflammatory responses.\n          <\/jats:p>","DOI":"10.1128\/iai.00526-16","type":"journal-article","created":{"date-parts":[[2016,10,25]],"date-time":"2016-10-25T02:30:02Z","timestamp":1477362602000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["SNAP23-Dependent Surface Translocation of Leukotriene B\n            <sub>4<\/sub>\n            (LTB\n            <sub>4<\/sub>\n            ) Receptor 1 Is Essential for NOX2-Mediated Exocytotic Degranulation in Human Mast Cells Induced by Trichomonas vaginalis-Secreted LTB\n            <sub>4<\/sub>"],"prefix":"10.1128","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2938-9630","authenticated-orcid":false,"given":"Arim","family":"Min","sequence":"first","affiliation":[{"name":"Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young Ah","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyeong Ah","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jamel","family":"El-Benna","sequence":"additional","affiliation":[{"name":"INSERM U1149, CNRS-ERL8252, Centre de Recherche sur l\u2019Inflammation, Laboratoire d\u2019Excellence Inflamex, Universit\u00e9 Paris-Diderot, Sorbonne Paris Cit\u00e9, Facult\u00e9 de M\u00e9decine Xavier Bichat, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myeong Heon","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Environmental Medical Biology, Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"Global Programme on AIDS Report Geneva","author":"WHO","year":"1995","unstructured":"WHO. 1995. 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