{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T08:04:31Z","timestamp":1696493071165},"reference-count":56,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,10]]},"abstract":"ABSTRACT<\/jats:title>\n \n Yersinia pestis<\/jats:named-content>\n , the causative agent of plague, evolved from the gastrointestinal pathogen\n Yersinia pseudotuberculosis<\/jats:named-content>\n . Both species have numerous type Va autotransporters, most of which appear to be highly conserved. In\n Y. pestis<\/jats:named-content>\n CO92, the autotransporter genes\n yapK<\/jats:italic>\n and\n yapJ<\/jats:italic>\n share a high level of sequence identity. By comparing\n yapK<\/jats:italic>\n and\n yapJ<\/jats:italic>\n to three homologous genes in\n Y. pseudotuberculosis<\/jats:named-content>\n IP32953 (YPTB0365, YPTB3285, and YPTB3286), we show that\n yapK<\/jats:italic>\n is conserved in\n Y. pseudotuberculosis<\/jats:named-content>\n , while\n yapJ<\/jats:italic>\n is unique to\n Y. pestis<\/jats:named-content>\n . All of these autotransporters exhibit >96% identity in the C terminus of the protein and identities ranging from 58 to 72% in their N termini. By extending this analysis to include homologous sequences from numerous\n Y. pestis<\/jats:named-content>\n and\n Y. pseudotuberculosis<\/jats:named-content>\n strains, we determined that these autotransporters cluster into a YapK (YPTB3285) class and a YapJ (YPTB3286) class. The YPTB3286-like gene of most\n Y. pestis<\/jats:named-content>\n strains appears to be inactivated, perhaps in favor of maintaining\n yapJ<\/jats:italic>\n . Since autotransporters are important for virulence in many bacterial pathogens, including\n Y. pestis<\/jats:named-content>\n , any change in autotransporter content should be considered for its impact on virulence. Using established mouse models of\n Y. pestis<\/jats:named-content>\n infection, we demonstrated that despite the high level of sequence identity,\n yapK<\/jats:italic>\n is distinct from\n yapJ<\/jats:italic>\n in its contribution to disseminated\n Y. pestis<\/jats:named-content>\n infection. In addition, a mutant lacking both of these genes exhibits an additive attenuation, suggesting nonredundant roles for\n yapJ<\/jats:italic>\n and\n yapK<\/jats:italic>\n in systemic\n Y. pestis<\/jats:named-content>\n infection. However, the deletion of the homologous genes in\n Y. pseudotuberculosis<\/jats:named-content>\n does not seem to impact the virulence of this organism in orogastric or systemic infection models.\n <\/jats:p>","DOI":"10.1128\/iai.00529-12","type":"journal-article","created":{"date-parts":[[2012,7,17]],"date-time":"2012-07-17T15:52:16Z","timestamp":1342540336000},"page":"3693-3705","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Evolution and Virulence Contributions of the Autotransporter Proteins YapJ and YapK of Yersinia pestis CO92 and Their Homologs in Y. pseudotuberculosis IP32953"],"prefix":"10.1128","volume":"80","author":[{"given":"Jonathan D.","family":"Lenz","sequence":"first","affiliation":[{"name":"Department of Genetics, University of North Carolina, Chapel Hill, North Carolina, USA"},{"name":"Division of Biology and Biomedical Sciences, Washington University, St. Louis, Missouri, USA"},{"name":"Department of Molecular Microbiology, Washington University, St. Louis, Missouri, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brenda R. 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