{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T03:58:53Z","timestamp":1777348733139,"version":"3.51.4"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2007,11,1]],"date-time":"2007-11-01T00:00:00Z","timestamp":1193875200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Bartonella henselae<\/jats:italic>\n            is a recently recognized pathogenic bacterium associated with cat scratch disease, bacillary angiomatosis, and bacillary peliosis. This study describes the cloning, sequencing, and characterization of an antigenic autotransporter gene from\n            <jats:italic>B. henselae<\/jats:italic>\n            . A cloned 6.0-kb BclI-EcoRI DNA fragment expresses a 120-kDa\n            <jats:italic>B. henselae<\/jats:italic>\n            protein immunoreactive with 21.2% of sera from patients positive for\n            <jats:italic>B. henselae<\/jats:italic>\n            immunoglobulin G antibodies by indirect immunofluorescence, with 97.3% specificity and no cross-reactivity with antibodies against various other organisms. DNA sequencing of the clone revealed one open reading frame of 4,320 bp with a deduced amino acid sequence that shows homology to the family of autotransporters. The autotransporters are a group of proteins that mediate their own export through the outer membrane and consist of a passenger region, the \u03b1-domain, and an outer membrane transporter region, the \u03b2-domain. The passenger domain shows homology to a family of pertactin-like adhesion proteins and contains seven, nearly identical 48-amino-acid repeats not found in any other bacterial or\n            <jats:italic>Bartonella<\/jats:italic>\n            DNA sequences. The passenger \u03b1-domain has a calculated molecular mass of 117 kDa, and the transporter \u03b2-domain has a calculated molecular mass of 36 kDa. The clone expresses a 120-kDa protein and a protein that migrates at approximately 38 kDa exclusively in the outer membrane protein fraction, suggesting that the 120-kDa passenger protein remains associated with the outer membrane after cleavage from the 36-kDa transporter.\n          <\/jats:p>","DOI":"10.1128\/iai.00533-07","type":"journal-article","created":{"date-parts":[[2007,9,5]],"date-time":"2007-09-05T00:53:54Z","timestamp":1188953634000},"page":"5255-5263","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Characterization of an Immunogenic Outer Membrane Autotransporter Protein, Arp, of\n            <i>Bartonella henselae<\/i>"],"prefix":"10.1128","volume":"75","author":[{"given":"Christine M.","family":"Litwin","sequence":"first","affiliation":[{"name":"Section of Clinical Immunology, Microbiology and Virology, Department of Pathology, University of Utah, Salt Lake City, Utah 84132"},{"name":"Associated Regional and University Pathologists (ARUP) Institute for Clinical and Experimental Pathology, Salt Lake City, Utah 84108"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mindy L.","family":"Rawlins","sequence":"additional","affiliation":[{"name":"Associated Regional and University Pathologists (ARUP) Institute for Clinical and Experimental Pathology, Salt Lake City, Utah 84108"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erica M.","family":"Swenson","sequence":"additional","affiliation":[{"name":"Associated Regional and University Pathologists (ARUP) Institute for Clinical and Experimental Pathology, Salt Lake City, Utah 84108"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(96)00330-7"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.9.2358-2365.1995"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.05.028"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.1.13-18.1992"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.8.1931-1937.1997"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00864-06"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.10.3554"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.6.3097-3105.2004"},{"key":"e_1_3_2_11_2","first-page":"1334","year":"1988","unstructured":"Cockerell, C. 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