{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T23:32:28Z","timestamp":1771457548985,"version":"3.50.1"},"reference-count":57,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2008,8,1]],"date-time":"2008-08-01T00:00:00Z","timestamp":1217548800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Inflammatory cytokines such as interleukin-1\u03b2 (IL-1\u03b2) are involved in the pathogenesis of periodontal diseases. A high individual variation in the levels of IL-1\u03b2 mRNA has been verified, which is possibly determined by genetic polymorphisms and\/or by the presence of periodontopathogens such as<jats:italic>Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola<\/jats:italic>, and<jats:italic>Aggregatibacter actinomycetemcomitans<\/jats:italic>. In this study, we investigated the role of an IL-1\u03b2 promoter single-nucleotide polymorphism at position 3954 [IL-1\u03b2(3954) SNP] and the presence of the periodontopathogens in the determination of the IL-1\u03b2 levels in the periodontal tissues of nonsmoking chronic periodontitis (CP) patients (<jats:italic>n<\/jats:italic>= 117) and control (C) subjects (<jats:italic>n<\/jats:italic>= 175) and the possible correlations with the clinical parameters of the disease. IL-1\u03b2(3954) SNP was investigated by restriction fragment length polymorphism, while the IL-1\u03b2 levels and the presence of the periodontopathogens were determined by real-time PCR. Similar frequencies of IL-1\u03b2(3954) SNP were found in the C and CP groups, in spite of a trend toward a higher incidence of T alleles in the CP group. The IL-1\u03b2(3954) SNP CT and TT genotypes, as well as<jats:italic>P. gingivalis, T. forsythia<\/jats:italic>, and<jats:italic>T. denticola<\/jats:italic>, were associated with higher IL-1\u03b2 levels and with higher values of the clinical parameters of disease severity. Concomitant analyses demonstrate that IL-1\u03b2(3954) and the red complex periodontopathogens were found to independently and additively modulate the levels of IL-1\u03b2 in periodontal tissues. Similarly, the concurrent presence of both factors was associated with increased scores of disease severity. IL-1\u03b2(3954) genotypes and red complex periodontopathogens, individually and additively, modulate the levels of IL-1\u03b2 in the diseased tissues of nonsmoking CP patients and, consequently, are potentially involved in the determination of the disease outcome.<\/jats:p>","DOI":"10.1128\/iai.00546-08","type":"journal-article","created":{"date-parts":[[2008,6,10]],"date-time":"2008-06-10T00:34:35Z","timestamp":1213058075000},"page":"3725-3734","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":62,"title":["An Interleukin-1\u03b2 (IL-1\u03b2) Single-Nucleotide Polymorphism at Position 3954 and Red Complex Periodontopathogens Independently and Additively Modulate the Levels of IL-1\u03b2 in Diseased Periodontal Tissues"],"prefix":"10.1128","volume":"76","author":[{"suffix":"Jr.","given":"Samuel B.","family":"Ferreira","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, School of Dentistry of Bauru, Sa\u0303o Paulo University (FOB\/USP), Bauru, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Paula F.","family":"Trombone","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, School of Medicine of Ribeira\u0303o Preto, Sa\u0303o Paulo University (FMRP\/USP), Ribeira\u0303o Preto, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos E.","family":"Repeke","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, School of Dentistry of Bauru, Sa\u0303o Paulo University (FOB\/USP), Bauru, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina R.","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, School of Medicine of Ribeira\u0303o Preto, Sa\u0303o Paulo University (FMRP\/USP), Ribeira\u0303o Preto, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Walter","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Periodontics, Dentistry School of the University of Ribeira\u0303o Preto (UNAERP), Ribeira\u0303o Preto, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos F.","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, School of Dentistry of Bauru, Sa\u0303o Paulo University (FOB\/USP), Bauru, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula Cristina","family":"Trevilatto","sequence":"additional","affiliation":[{"name":"School of Dentistry, Pontifical Catholic University of Parana\u0301 (PUC-PR), Curitiba, PR, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario J.","family":"A\u0301vila-Campos","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Institute of Biomedical Sciences (ICB\/USP), Sa\u0303o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana Paula","family":"Campanelli","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, School of Dentistry of Bauru, Sa\u0303o Paulo University (FOB\/USP), Bauru, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joa\u0303o S.","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology, School of Medicine of Ribeira\u0303o Preto, Sa\u0303o Paulo University (FMRP\/USP), Ribeira\u0303o Preto, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo P.","family":"Garlet","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, School of Dentistry of Bauru, Sa\u0303o Paulo University (FOB\/USP), Bauru, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.050212"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2005.05.015"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gene.6363766"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0501.2004.01091.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gene.6364032"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddi469"},{"key":"e_1_3_2_8_2","first-page":"450","volume":"1","year":"1992","unstructured":"di Giovine, F. 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