{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T13:52:31Z","timestamp":1773928351410,"version":"3.50.1"},"reference-count":59,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:00:00Z","timestamp":1765843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:00:00Z","timestamp":1765843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2025,12,16]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:bold>\u2003<\/jats:bold>\n                      <jats:italic toggle=\"yes\">Chlamydia trachomatis<\/jats:italic>\n                      (\n                      <jats:italic toggle=\"yes\">C.t<\/jats:italic>\n                      .), the leading bacterial cause of sexually transmitted infections, replicates within a unique intracellular compartment called the inclusion, which is modified by secreted proteins known as inclusion membrane (Inc) proteins. Here, we further characterize CpoS, an Inc protein previously shown to be critical for bacterial replication and inclusion development. We demonstrate that CpoS directly binds multiple coiled-coil region-containing Incs and engages Rab GTPases at a separate site. Notably, CpoS-InaC interactions facilitate the recruitment of select Arf GTPases to the inclusion membrane, while Rab recruitment occurs independently of these interactions. Biochemical and biophysical analyses revealed that Incs self-oligomerize to form higher-ordered structures, with CpoS adopting a tetrameric conformation resembling that of eukaryotic SNARE proteins. We propose that these assemblies serve as scaffolds to orchestrate vesicle docking, tethering, and fusion. Our findings highlight the intricate interplay between bacterial and host factors, revealing how\n                      <jats:italic toggle=\"yes\">C.t<\/jats:italic>\n                      . leverages both Inc-Inc interactions and host protein engagement to manipulate vesicular trafficking and sustain infection.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/iai.00548-25","type":"journal-article","created":{"date-parts":[[2025,11,18]],"date-time":"2025-11-18T13:00:15Z","timestamp":1763470815000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["CpoS-Inc interactions facilitate host cell modulation during\n                    <i>Chlamydia trachomatis<\/i>\n                    infection"],"prefix":"10.1128","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5169-9614","authenticated-orcid":false,"given":"Xavier","family":"Tijerina","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Iowa Carver College of Medicine","place":["Iowa City, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.A.","family":"Jabeena","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Iowa Carver College of Medicine","place":["Iowa City, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0855-7041","authenticated-orcid":true,"given":"Robert","family":"Faris","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Iowa Carver College of Medicine","place":["Iowa City, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Xu","sequence":"additional","affiliation":[{"name":"Protein and Crystallography Facility, University of Iowa Carver College of Medicine","place":["Iowa City, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parker","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Iowa Carver College of Medicine","place":["Iowa City, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas J.","family":"Schnicker","sequence":"additional","affiliation":[{"name":"Protein and Crystallography Facility, University of Iowa Carver College of Medicine","place":["Iowa City, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5126-2164","authenticated-orcid":true,"given":"Mary M.","family":"Weber","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Iowa Carver College of Medicine","place":["Iowa City, USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2021.03.043"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.30"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/652397"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(18)30421-2"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.18502\/ijph.v51i6.9705"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01640708"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.18502\/ijrm.v17i9.5093"},{"key":"e_1_3_4_9_2","first-page":"285","article-title":"The prevalence of Chlamydia trachomatis in patients who remained symptomatic after completion of sexually transmitted infection treatment","volume":"11","author":"Afrakhteh M","year":"2011","unstructured":"Afrakhteh M, Mahdavi A, Beyhaghi H, Moradi A, Gity S, Zafargandi S, Zonoubi Z. 2011. 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