{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T22:34:01Z","timestamp":1793226841427,"version":"4.5.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Erysipelothrix rhusiopathiae<\/jats:named-content>\n            , the causative agent of swine erysipelas, is a facultative intracellular Gram-positive bacterium. It has been shown that animals immunized with a filtrate from\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. rhusiopathiae<\/jats:named-content>\n            cultures are protected against lethal challenge. In this study, we identified and characterized the extracellular proteins of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. rhusiopathiae<\/jats:named-content>\n            to search for novel vaccine antigens. A concentrated culture supernatant from the\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. rhusiopathiae<\/jats:named-content>\n            Fujisawa strain, which has been found to induce protection in mice, was analyzed using two-dimensional electrophoresis. From more than 40 confirmed protein spots, 16 major protein spots were selected and subjected to N-terminal amino acid sequence determination, and 14 protein spots were successfully identified. The identified proteins included housekeeping proteins and other metabolic enzymes. We searched for surface-localized proteins by analyzing the genomes of two\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. rhusiopathiae<\/jats:named-content>\n            strains: Fujisawa and ATCC 19414. Genome analysis revealed that the ATCC 19414 strain has three putative surface-exposed\n            <jats:italic>c<\/jats:italic>\n            holine-\n            <jats:italic>b<\/jats:italic>\n            inding\n            <jats:italic>p<\/jats:italic>\n            roteins (CBPs): CbpA, CbpB, and CbpC. Each CBP contains a putative choline-binding domain. The CbpC gene is mutated in Fujisawa, becoming a nonfunctional pseudogene. Immunogold electron microscopy confirmed that CbpA and CbpB, as well as the majority of the metabolic enzymes examined, are associated with the cell surface of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">E. rhusiopathiae<\/jats:named-content>\n            Fujisawa. Immunization with recombinant CbpB, but not with other recombinant CBPs or metabolic enzymes, protected mice against lethal challenge. A phagocytosis assay revealed that antiserum against CbpB promoted opsonin-mediated phagocytosis by murine macrophages\n            <jats:italic>in vitro<\/jats:italic>\n            . The protective capabilities of CbpB were confirmed in pigs, suggesting that CbpB could be used as a vaccine antigen.\n          <\/jats:p>","DOI":"10.1128\/iai.00549-13","type":"journal-article","created":{"date-parts":[[2013,9,10]],"date-time":"2013-09-10T01:53:55Z","timestamp":1378778035000},"page":"4333-4340","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Characterization and Identification of a Novel Candidate Vaccine Protein through Systematic Analysis of Extracellular Proteins of Erysipelothrix rhusiopathiae"],"prefix":"10.1128","volume":"81","author":[{"given":"Fang","family":"Shi","sequence":"first","affiliation":[{"name":"National Institute of Animal Health, Tsukuba, Ibaraki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohsuke","family":"Ogawa","sequence":"additional","affiliation":[{"name":"National Institute of Animal Health, Tsukuba, Ibaraki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akiyuki","family":"Sano","sequence":"additional","affiliation":[{"name":"Kyoto Biken Laboratories, Uji, Kyoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoyuki","family":"Harada","sequence":"additional","affiliation":[{"name":"National Institute of Animal Health, Tsukuba, Ibaraki, Japan"},{"name":"Department of Veterinary Pathology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiro","family":"Hirota","sequence":"additional","affiliation":[{"name":"National Institute of Animal Health, Tsukuba, Ibaraki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Eguchi","sequence":"additional","affiliation":[{"name":"National Institute of Animal Health, Tsukuba, Ibaraki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eiji","family":"Oishi","sequence":"additional","affiliation":[{"name":"Kyoto Biken Laboratories, Uji, Kyoto, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiro","family":"Shimoji","sequence":"additional","affiliation":[{"name":"National Institute of Animal Health, Tsukuba, Ibaraki, Japan"},{"name":"Research Institute for Biological Sciences, Tokyo University of Science, Noda, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.12.6455-6467.1989"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01500-10"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.048983-0"},{"key":"e_1_3_3_5_2","first-page":"475","volume-title":"Diseases of swine","author":"Wood RL","year":"1992","unstructured":"WoodRL. 1992. Erysipelas, p 475\u2013486. In LemanADStrawBEMengelingWLD'AllaireSTaylorDJ (ed), Diseases of swine, 7th ed. Iowa State University Press, Ames, IA.","edition":"7"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00206-10"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2010.09.012"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2006.02.015"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063870802000313"},{"key":"e_1_3_3_10_2","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1128\/9781555816513","volume-title":"Gram-positive pathogens","author":"Fischetti VA","year":"2006","unstructured":"FischettiVA. 2006. Surface proteins on gram-positive bacteria, p 12\u201325. In FischettiVANovickRPFerrettiJJPortnoyDARoodJI (ed), Gram-positive pathogens, 2nd ed. ASM Press, Washington, DC.","edition":"2"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.63.1.174-229.1999"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28610-0"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-10-190"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2009.00172.x"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.65.2.187-207.2001"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1997.mmi494.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.176.10.2976-2985.1994"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(02)02351-X"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00283"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.23.14503"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.176.2.415"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.10.6237-6248.2005"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00635-12"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(92)90095-B"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(95)92533-H"},{"key":"e_1_3_3_26_2","first-page":"239","article-title":"Cross protection of mice and swine inoculated with culture filtrate of attenuated Erysipelothrix rhusiopathiae and challenge exposed to strains of various serovars","volume":"48","author":"Sawada T","year":"1987","unstructured":"SawadaTTakahashiT. 1987. Cross protection of mice and swine inoculated with culture filtrate of attenuated Erysipelothrix rhusiopathiae and challenge exposed to strains of various serovars. Am. J. Vet. Res. 48:239\u2013242.","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.1.4.380-386.1970"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.1.4.387-393.1970"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.58.9.3116-3121.1990"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1006\/mpat.1998.0216"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.4.1646-1651.1999"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.9.2739-2748.2003"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00342-12"},{"key":"e_1_3_3_34_2","first-page":"102","article-title":"Bioinformatics tool for genomic era: a step towards the in silico experiments\u2014focused on molecular cloning","volume":"7","author":"Ohyama A","year":"2006","unstructured":"OhyamaAKurokawaKEnaiKSaitohHKanayaSAltaf-Ul-AminMOgasawaraN. 2006. Bioinformatics tool for genomic era: a step towards the in silico experiments\u2014focused on molecular cloning. J. Comp. Aid Chem. 7:102\u2013115.","journal-title":"J. Comp. Aid Chem."},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.1.226-232.2002"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.7.2806-2810.1994"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.9.4376-4382.1999"},{"key":"e_1_3_3_38_2","first-page":"279","article-title":"Enzymology of bacterial lysine biosynthesis","volume":"72","author":"Scapin G","year":"1998","unstructured":"ScapinGBlanchardJS. 1998. Enzymology of bacterial lysine biosynthesis. Adv. Enzymol. Relat. Areas Mol. Biol. 72:279\u2013324.","journal-title":"Adv. Enzymol. Relat. Areas Mol. Biol."}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00549-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00549-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T20:14:39Z","timestamp":1646424879000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00549-13"}},"subtitle":[],"editor":[{"suffix":"Jr.","given":"J. F.","family":"Urban","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,12]]},"references-count":37,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["10.1128\/IAI.00549-13"],"URL":"https:\/\/doi.org\/10.1128\/iai.00549-13","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12]]},"assertion":[{"value":"2013-05-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-08-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-11-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}