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In this study, we found that inactivation of\n            <jats:italic>cas9<\/jats:italic>\n            caused reduced adhesion and intracellular survival of the piscine\n            <jats:named-content content-type=\"genus-species\">Streptococcus agalactiae<\/jats:named-content>\n            strain GD201008-001 and significantly decreased the virulence of this strain in zebrafish and mice. Further investigation indicated that the\n            <jats:italic>regR<\/jats:italic>\n            transcriptional regulator was upregulated in the \u0394\n            <jats:italic>cas9<\/jats:italic>\n            mutant. As\n            <jats:italic>regR<\/jats:italic>\n            mediates the repression of hyaluronidase, a critical factor involved in opening the blood-brain barrier (BBB) in mice,\n            <jats:italic>cas9<\/jats:italic>\n            -mediated repression of\n            <jats:italic>regR<\/jats:italic>\n            transcription is important for\n            <jats:named-content content-type=\"genus-species\">S. agalactiae<\/jats:named-content>\n            to open the BBB and thereby cause meningitis in animals. This study expands our understanding of endogenous gene regulation mediated by CRISPR-Cas systems in bacteria.\n          <\/jats:p>","DOI":"10.1128\/iai.00552-17","type":"journal-article","created":{"date-parts":[[2017,12,11]],"date-time":"2017-12-11T16:15:30Z","timestamp":1513008930000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["<i>cas9<\/i>\n            Enhances Bacterial Virulence by Repressing the\n            <i>regR<\/i>\n            Transcriptional Regulator in Streptococcus agalactiae"],"prefix":"10.1128","volume":"86","author":[{"given":"Ke","family":"Ma","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Cao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su","family":"Luo","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaofei","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangjin","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengping","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjie","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/432804"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/SMJ.0b013e318164e0e3"},{"key":"e_1_3_3_4_2","first-page":"429","article-title":"Streptococcus agalactiae mastitis: a review","volume":"38","author":"Keefe GP","year":"1997","unstructured":"Keefe GP. 1997. 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