{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T20:58:55Z","timestamp":1785531535775,"version":"3.56.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2006,10,1]],"date-time":"2006-10-01T00:00:00Z","timestamp":1159660800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The human pathogen\n            <jats:italic>Streptococcus pneumoniae<\/jats:italic>\n            contains genes for a putative polyamine ABC transporter which are organized in an operon and designated\n            <jats:italic>potABCD<\/jats:italic>\n            . Polyamine transport protein D (PotD) is an extracellular protein which binds polyamines and possibly other structurally related molecules. PotD has been shown to contribute to virulence in both a murine sepsis model and a pneumonia model with capsular type 3 pneumococci. The protective efficacy of recombinant PotD was evaluated by active immunization and intravenous challenge with capsular type 3 pneumococci in CBA\/N mice. Immunized mice had 91.7% survival following lethal pneumococcal challenge, compared with 100% mortality in the control group. Immunized animals had high-titer anti-PotD antibodies following three immunizations with alum. Protection in a sepsis model was also seen after passive administration of rabbit antiserum raised against PotD (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.004). These results suggest that antibodies to PotD confer protection against invasive pneumococcal disease and that this protein should be studied further as a potential vaccine candidate for protection against invasive pneumococcal infections.\n          <\/jats:p>","DOI":"10.1128\/iai.00553-06","type":"journal-article","created":{"date-parts":[[2006,9,20]],"date-time":"2006-09-20T23:06:35Z","timestamp":1158793595000},"page":"5888-5892","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":43,"title":["Immunization with Polyamine Transport Protein PotD Protects Mice against Systemic Infection with\n            <i>Streptococcus pneumoniae<\/i>"],"prefix":"10.1128","volume":"74","author":[{"given":"P.","family":"Shah","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Mississippi Medical Center, 2500 N. State Street, Jackson, Mississippi 39216"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Swiatlo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Mississippi Medical Center, 2500 N. 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