{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T23:42:45Z","timestamp":1790379765537,"version":"4.1.0"},"reference-count":58,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2009,10,1]],"date-time":"2009-10-01T00:00:00Z","timestamp":1254355200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2009,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Toll-like receptors and Nod-like receptors (NLR) play an important role in sensing invading microorganisms for pathogen clearance and eliciting adaptive immunity for protection against rechallenge. Nod1 and Nod2, members of the NLR family, are capable of detecting bacterial peptidoglycan motifs in the host cytosol for triggering proinflammatory cytokine production. In the current study, we sought to determine if Nod1\/Nod2 are involved in sensing\n            <jats:italic>Bacillus anthracis<\/jats:italic>\n            infection and eliciting protective immune responses. Using mice deficient in both Nod1 and Nod2 proteins, we showed that Nod1\/Nod2 are involved in detecting\n            <jats:italic>B. anthracis<\/jats:italic>\n            for production of tumor necrosis factor alpha, interleukin-1\u03b1 (IL-1\u03b1), IL-1\u03b2, CCL5, IL-6, and KC. Proinflammatory responses were higher when cells were exposed to viable spores than when they were exposed to irradiated spores, indicating that recognition of vegetative bacilli through Nod1\/Nod2 is significant. We also identify a critical role for Nod1\/Nod2 in priming responses after\n            <jats:italic>B. anthracis<\/jats:italic>\n            aerosol exposure, as mice deficient in Nod1\/Nod2 were impaired in their ability to mount an anamnestic antibody response and were more susceptible to secondary lethal challenge than wild-type mice.\n          <\/jats:p>","DOI":"10.1128\/iai.00563-09","type":"journal-article","created":{"date-parts":[[2009,7,21]],"date-time":"2009-07-21T00:58:43Z","timestamp":1248137923000},"page":"4529-4537","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Nod1\/Nod2-Mediated Recognition Plays a Critical Role in Induction of Adaptive Immunity to Anthrax after Aerosol Exposure"],"prefix":"10.1128","volume":"77","author":[{"given":"Crystal L.","family":"Loving","sequence":"first","affiliation":[{"name":"Laboratory of Respiratory and Special Pathogens"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Osorio","sequence":"additional","affiliation":[{"name":"Laboratory of Enteric and Sexually Transmitted Diseases, Division of Bacterial, Parasitic and Allergenic Products, CBER, FDA, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Gi","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pathology and Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Nun\u0303ez","sequence":"additional","affiliation":[{"name":"Department of Pathology and Comprehensive Cancer Center, University of Michigan Medical School, Ann Arbor, Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Molly A.","family":"Hughes","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Internal Medicine, University of Virginia Health Sciences System, Charlottesville, Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tod J.","family":"Merkel","sequence":"additional","affiliation":[{"name":"Laboratory of Respiratory and Special Pathogens"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01794"},{"key":"e_1_3_2_3_2","first-page":"385","volume":"28","year":"1947","unstructured":"Barnes, J. 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