{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:30:45Z","timestamp":1787016645944,"version":"build-2736575974"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["5R01AI018000-33"],"award-info":[{"award-number":["5R01AI018000-33"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009455","name":"West Virginia University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009455","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:named-content content-type=\"genus-species\">Bordetella pertussis<\/jats:named-content>\n                    is a human pathogen that can infect the respiratory tract and cause the disease known as whooping cough.\n                    <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n                    uses pertussis toxin (PT) and adenylate cyclase toxin (ACT) to kill and modulate host cells to allow the pathogen to survive and persist.\n                    <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n                    encodes many uncharacterized transcription factors, and very little is known about their functions. RpoE is a sigma factor which, in other bacteria, responds to oxidative, heat, and other environmental stresses. RseA is a negative regulator of RpoE that sequesters the sigma factor to regulate gene expression based on conditions. In\n                    <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n                    , deletion of the\n                    <jats:italic>rseA<\/jats:italic>\n                    gene results in high transcriptional activity of RpoE and large amounts of secretion of ACT. By comparing parental\n                    <jats:named-content content-type=\"genus-species\">B. pertussis<\/jats:named-content>\n                    to an\n                    <jats:italic>rseA<\/jats:italic>\n                    gene deletion mutant (PM18), we sought to characterize the roles of RpoE in virulence and determine the regulon of genes controlled by RpoE. Despite high expression of ACT, the\n                    <jats:italic>rseA<\/jats:italic>\n                    mutant strain did not infect the murine airway as efficiently as the parental strain and PM18 was killed more readily when inside phagocytes. RNA sequencing analysis was performed and 263 genes were differentially regulated by RpoE, and surprisingly, the\n                    <jats:italic>rseA<\/jats:italic>\n                    mutant strain where RpoE activity was elevated expressed very little pertussis toxin. Western blots and proteomic analysis corroborated the inverse relationship of PT to ACT expression in the high-RpoE-activity\n                    <jats:italic>rseA<\/jats:italic>\n                    deletion strain. Our data suggest that RpoE can modulate PT and ACT expression indirectly through unidentified mechanisms in response to conditions.\n                  <\/jats:p>","DOI":"10.1128\/iai.00565-16","type":"journal-article","created":{"date-parts":[[2016,11,14]],"date-time":"2016-11-14T21:50:31Z","timestamp":1479160231000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Modulation of Pertussis and Adenylate Cyclase Toxins by Sigma Factor RpoE in Bordetella pertussis"],"prefix":"10.1128","volume":"85","author":[{"given":"Mariette","family":"Barbier","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology, and Cell Biology, West Virginia University, Morgantown, West Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dylan T.","family":"Boehm","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Cell Biology, West Virginia University, Morgantown, West Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emel","family":"Sen-Kilic","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Cell Biology, West Virginia University, Morgantown, West Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Bonnin","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Cell Biology, West Virginia University, Morgantown, West Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theo","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Cell Biology, West Virginia University, Morgantown, West Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Casey","family":"Hoffman","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Gray","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Hewlett","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3140-402X","authenticated-orcid":false,"given":"F. Heath","family":"Damron","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Cell Biology, West Virginia University, Morgantown, West Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3235"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1200850"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.06310-11"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jis443"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit639"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/589753"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.5.2698-2703.2005"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-8-220"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/150.2.219"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.9.4367-4373.1998"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.3821741.x"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-12-179"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/2049-632X.12061"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506577102"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.17.5692-5698.2004"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.19.5670-5679.1995"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2008.06.004"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01132-10"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0105011"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.5.1775-1785.2006"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00345-13"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.4.1153-1164.1993"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2012.08049.x"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.1.54-60.1996"},{"key":"e_1_3_3_26_2","first-page":"731","article-title":"Le microbe de la coqueluche","volume":"20","author":"Bordet J","year":"1906","unstructured":"Bordet J Gengou O . 1906. Le microbe de la coqueluche. Ann Inst Pasteur (Paris)20:731\u2013741.","journal-title":"Ann Inst Pasteur (Paris)"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-63-2-211"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1227"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/biostatistics\/kxm030"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btl567"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq973"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq249"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.5.2090-2095.1999"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1981.tb06245.x"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.3791\/2437"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr060161n"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00565-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00565-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T05:31:51Z","timestamp":1646458311000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00565-16"}},"subtitle":[],"editor":[{"given":"Vincent B.","family":"Young","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,1]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,1]]}},"alternative-id":["10.1128\/IAI.00565-16"],"URL":"https:\/\/doi.org\/10.1128\/iai.00565-16","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1]]},"assertion":[{"value":"2016-07-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-11-07","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-12-29","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00565-16"}}