{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T11:17:43Z","timestamp":1792667863046,"version":"4.5.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2008,10,1]],"date-time":"2008-10-01T00:00:00Z","timestamp":1222819200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Mycoplasma pneumoniae<\/jats:italic>\n                    is a frequent cause of community-acquired bacterial respiratory infections in children and adults. In the present study, using a proteomic approach, we studied the effects of\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    infection on the protein expression profile of A549 human lung carcinoma cells.\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    infection induced changes in the expression of cellular proteins, in particular a group of proteins involved in the oxidative stress response, such as glucose-6-phosphate 1-dehydrogenase, NADH dehydrogenase (ubiquinone) Fe-S protein 2, and ubiquinol-cytochrome\n                    <jats:italic>c<\/jats:italic>\n                    reductase complex core protein I mitochondrial precursor. The oxidative status of\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    -infected cells was evaluated, and the results revealed that\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    infection indeed caused generation of reactive oxygen species (ROS). It was further shown that\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    infection also induced DNA double-strand breaks, as demonstrated by the formation of \u03b3H2AX foci. On the other hand, an ROS scavenger,\n                    <jats:italic>N<\/jats:italic>\n                    -acetylcysteine, could inhibit the ROS generation, as well as decrease \u03b3H2AX focus formation. This is the first report showing that\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    infection can directly induce DNA damage, at least partially, through the generation of ROS, and thus this report strengthens the powerful application of proteomics in the study of the pathogenesis of\n                    <jats:italic>M. pneumoniae<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1128\/iai.00575-08","type":"journal-article","created":{"date-parts":[[2008,7,28]],"date-time":"2008-07-28T20:44:07Z","timestamp":1217277847000},"page":"4405-4413","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":65,"title":["<i>Mycoplasma pneumoniae<\/i>\n                    Infection Induces Reactive Oxygen Species and DNA Damage in A549 Human Lung Carcinoma Cells"],"prefix":"10.1128","volume":"76","author":[{"given":"Gongping","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Toxicology, Zhejiang University School of Public Health, Hangzhou, Zhejiang, 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuefeng","family":"Xu","sequence":"additional","affiliation":[{"name":"The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingshuo","family":"Wang","sequence":"additional","affiliation":[{"name":"The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyun","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Toxicology, Zhejiang University School of Public Health, Hangzhou, Zhejiang, 310058, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhimin","family":"Chen","sequence":"additional","affiliation":[{"name":"The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310008, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Toxicology, Zhejiang University School of Public Health, Hangzhou, Zhejiang, 310058, China"},{"name":"National Key Laboratory for Infectious Disease Diagnosis and Therapy, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310008, China"},{"name":"Zhejiang California International NanoSystems Institute, Hangzhou, Zhejiang, 310030, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.52.1.240-244.1986"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.48.2.552-555.1985"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI111279"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.56.3.570-576.1988"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.2570100603"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genom.4.070802.110305"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrgentox.2007.01.003"},{"key":"e_1_3_2_9_2","first-page":"1277","volume":"72","year":"2005","unstructured":"Frank, J. 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