{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T03:14:09Z","timestamp":1791861249888,"version":"4.3.4"},"reference-count":105,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T00:00:00Z","timestamp":1509494400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["5 T32 AI055413-14"],"award-info":[{"award-number":["5 T32 AI055413-14"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["K12 GM084897"],"award-info":[{"award-number":["K12 GM084897"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["K12 GM084897"],"award-info":[{"award-number":["K12 GM084897"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI126354"],"award-info":[{"award-number":["AI126354"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007915","name":"Baylor College of Medicine","doi-asserted-by":"publisher","award":["Seed Funds"],"award-info":[{"award-number":["Seed Funds"]}],"id":[{"id":"10.13039\/100007915","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The translocation of bacteria across the intestinal epithelium of immunocompromised patients can lead to bacteremia and life-threatening sepsis. Extraintestinal pathogenic\n                    <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n                    (ExPEC), so named because this pathotype infects tissues distal to the intestinal tract, is a frequent cause of such infections, is often multidrug resistant, and chronically colonizes a sizable portion of the healthy population. Although several virulence factors and their roles in pathogenesis are well described for ExPEC strains that cause urinary tract infections and meningitis, they have not been linked to translocation through intestinal barriers, a fundamentally distant yet important clinical phenomenon. Using untransformed\n                    <jats:italic>ex situ<\/jats:italic>\n                    human intestinal enteroids and transformed Caco-2 cells, we report that ExPEC strain CP9 binds to and invades the intestinal epithelium. ExPEC harboring a deletion of the gene encoding the mannose-binding type 1 pilus tip protein FimH demonstrated reduced binding and invasion compared to strains lacking known\n                    <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n                    virulence factors. Furthermore, in a murine model of chemotherapy-induced translocation, ExPEC lacking\n                    <jats:italic>fimH<\/jats:italic>\n                    colonized at levels comparable to that of the wild type but demonstrated a statistically significant reduction in translocation to the kidneys, spleen, and lungs. Collectively, this study indicates that FimH is important for ExPEC translocation, suggesting that the type 1 pilus is a therapeutic target for the prevention of this process. Our study also highlights the use of human intestinal enteroids in the study of enteric diseases.\n                  <\/jats:p>","DOI":"10.1128\/iai.00581-17","type":"journal-article","created":{"date-parts":[[2017,8,14]],"date-time":"2017-08-14T20:50:19Z","timestamp":1502743819000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":54,"title":["Role for FimH in Extraintestinal Pathogenic Escherichia coli Invasion and Translocation through the Intestinal Epithelium"],"prefix":"10.1128","volume":"85","author":[{"given":"Nina M.","family":"Poole","sequence":"first","affiliation":[{"name":"Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina I.","family":"Green","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anubama","family":"Rajan","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luz E.","family":"Vela","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi-Lei","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary K.","family":"Estes","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony W.","family":"Maresso","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/116.4.523"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.23.2.403-411.1979"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/157.5.1032"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(00)88906-4"},{"key":"e_1_3_3_6_2","volume-title":"The scientific basis of the probiotic concept","author":"Berg RD","year":"1992","unstructured":"Berg RD . 1992. The scientific basis of the probiotic concept, vol 3. Chapman and Hall, London, United Kingdom."},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.33.3.854-861.1981"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.27.2.461-467.1980"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.7.2874-2884.1994"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(82)90115-9"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01577729"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(86)90638-4"},{"key":"e_1_3_3_13_2","first-page":"191","article-title":"Hemorrhagic shock-induced bacterial translocation is reduced by xanthine oxidase inhibition or inactivation","volume":"104","author":"Deitch EA","year":"1988","unstructured":"Deitch EA Bridges W Baker J Ma JW Ma L Grisham MB Granger DN Specian RD Berg R . 1988. Hemorrhagic shock-induced bacterial translocation is reduced by xanthine oxidase inhibition or inactivation. Surgery104:191\u2013198.","journal-title":"Surgery"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/bmb\/ldh029"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.45.2.223"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.42.1.29"},{"key":"e_1_3_3_17_2","volume-title":"Internal medicine: handbook for clinicians, resident survival guide","author":"Huang E","year":"2000","unstructured":"Huang E . 2000. Internal medicine: handbook for clinicians, resident survival guide. Scrub Hill Press, Arlington, VA."},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1086\/421946"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-013-1886-9"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1086\/315418"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro818"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2005.07.005"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1086\/319757"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01509-06"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(08)70041-0"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2010.190"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02824-14"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2011.09.006"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2005.07.007"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00009"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1067\/msy.2002.116408"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.5.2132-2134.1994"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2006.00140.x"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0580-9517(02)31009-2"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0029968"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.12.7156-7160.2002"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00656-06"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.6.3954-3964.2001"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.12.2803"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.7.4572-4579.2001"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1084550"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00952.x"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00323-09"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2011.07.050"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01930-15"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1177\/1535370214529398"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2015.10.001"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00516-10"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004630-199405000-00002"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00684-15"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri2850"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2012.03879.x"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-005-0077-8"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ273"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiv279"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.5495\/wjcid.v1.i1.17"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.34.3.708-711.1981"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.53.3.693-696.1986"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/156.4.567"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.25.12.2253-2257.1987"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.6.1656-1662.1989"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.7.3303-3310.1998"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.2.1387-1393.2006"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02915.x"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0902179106"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000118402.01034.fb"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.18.9827"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04415.x"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm3010338"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1002\/cmdc.201600006"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt467"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit245"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.15.8922"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01963-06"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04985.x"},{"key":"e_1_3_3_76_2","first-page":"339","article-title":"Epidemiologic investigation of extra-intestinal pathogenic E. coli (ExPEC) based on PCR phylogenetic group and fimH single nucleotide polymorphisms (SNPs) in China","volume":"2","author":"Abdallah KS","year":"2011","unstructured":"Abdallah KS Cao Y Wei DJ . 2011. Epidemiologic investigation of extra-intestinal pathogenic E. coli (ExPEC) based on PCR phylogenetic group and fimH single nucleotide polymorphisms (SNPs) in China. Int J Mol Epidemiol Genet2:339\u2013353.","journal-title":"Int J Mol Epidemiol Genet"},{"key":"e_1_3_3_77_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.06663-11"},{"key":"e_1_3_3_78_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.28.17880"},{"key":"e_1_3_3_79_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1315203110"},{"key":"e_1_3_3_80_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature22972"},{"key":"e_1_3_3_81_2","doi-asserted-by":"publisher","DOI":"10.1007\/s005209900095"},{"key":"e_1_3_3_82_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00978.x"},{"key":"e_1_3_3_83_2","doi-asserted-by":"publisher","DOI":"10.1038\/86373"},{"key":"e_1_3_3_84_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08529"},{"key":"e_1_3_3_85_2","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevImmunol.v23.i56.20"},{"key":"e_1_3_3_86_2","doi-asserted-by":"publisher","DOI":"10.1186\/1478-811X-11-72"},{"key":"e_1_3_3_87_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.18.9630"},{"key":"e_1_3_3_88_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M600877200"},{"key":"e_1_3_3_89_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.114.22.4095"},{"key":"e_1_3_3_90_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M400769200"},{"key":"e_1_3_3_91_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2007.05.007"},{"key":"e_1_3_3_92_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030100"},{"key":"e_1_3_3_93_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2002.00166.x"},{"key":"e_1_3_3_94_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M805010200"},{"key":"e_1_3_3_95_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1572"},{"key":"e_1_3_3_96_2","doi-asserted-by":"publisher","DOI":"10.1086\/514006"},{"key":"e_1_3_3_97_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01696.x"},{"key":"e_1_3_3_98_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.23.7617-7623.1993"},{"key":"e_1_3_3_99_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.06388-11"},{"key":"e_1_3_3_100_2","first-page":"L632","article-title":"Surfactant dysfunction and lung injury due to the E. coli virulence factor hemolysin in a rat pneumonia model","volume":"292","author":"Russo TA","year":"2007","unstructured":"Russo TA Wang Z Davidson BA Genagon SA Beanan JM Olson R Holm BA Knight PR III Chess PR Notter RH . 2007. Surfactant dysfunction and lung injury due to the E. coli virulence factor hemolysin in a rat pneumonia model. Am J Physiol292:L632\u2013L643.","journal-title":"Am J Physiol"},{"key":"e_1_3_3_101_2","doi-asserted-by":"publisher","DOI":"10.1086\/507305"},{"key":"e_1_3_3_102_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2013.02.031"},{"key":"e_1_3_3_103_2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2013-306651"},{"key":"e_1_3_3_104_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaf5211"},{"key":"e_1_3_3_105_2","first-page":"3919","article-title":"New methodological approach to induce a differentiation phenotype in Caco-2 cells prior to post-confluence stage","volume":"27","author":"Ferraretto A","year":"2007","unstructured":"Ferraretto A Gravaghi C Donetti E Cosentino S Donida BM Bedoni M Lombardi G Fiorilli A Tettamanti G . 2007. New methodological approach to induce a differentiation phenotype in Caco-2 cells prior to post-confluence stage. Anticancer Res27:3919\u20133926.","journal-title":"Anticancer Res"},{"key":"e_1_3_3_106_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.892001"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00581-17","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00581-17","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T05:28:08Z","timestamp":1646458088000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00581-17"}},"subtitle":[],"editor":[{"given":"Andreas J.","family":"B\u00e4umler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,11]]},"references-count":105,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2017,11]]}},"alternative-id":["10.1128\/IAI.00581-17"],"URL":"https:\/\/doi.org\/10.1128\/iai.00581-17","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11]]},"assertion":[{"value":"2017-08-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-08-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-10-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00581-17"}}