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While these species have led to countless breakthroughs in our understanding of infectious disease, there are undoubtedly important biological processes that are missed by limiting studies to these two vertebrate species. For instance, it is well-established that a common deermouse in North America,\n                      <jats:italic toggle=\"yes\">Peromyscus leucopus<\/jats:italic>\n                      , has unique interactions with microbes, which likely shape its ability to serve as a critical reservoir for numerous zoonotic pathogens, including a Lyme disease spirochete,\n                      <jats:italic toggle=\"yes\">Borrelia burgdorferi<\/jats:italic>\n                      . In this work, we expand the immunological toolkit to study\n                      <jats:italic toggle=\"yes\">P. leucopus<\/jats:italic>\n                      biology by performing the first differentiation of deermouse bone marrow to macrophages using\n                      <jats:italic toggle=\"yes\">P. leucopus<\/jats:italic>\n                      M-CSF producing HEK293T cells. We find that\n                      <jats:italic toggle=\"yes\">P. leucopus<\/jats:italic>\n                      BMDMs generated through this method behave broadly very similarly to C57BL\/6J macrophages generated with the L-929 supernatant, although RNA sequencing revealed modest differences in transcriptomic responses to\n                      <jats:italic toggle=\"yes\">B. burgdorferi<\/jats:italic>\n                      and lipopolysaccharide. In particular, differences in Il-10 induction and caspase expression were observed between the species.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/iai.00581-24","type":"journal-article","created":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T09:00:19Z","timestamp":1748336419000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Differentiating\n                    <i>Peromyscus leucopus<\/i>\n                    bone marrow-derived macrophages for characterization of responses to\n                    <i>Borrelia burgdorferi<\/i>\n                    and lipopolysaccharide"],"prefix":"10.1128","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2260-8546","authenticated-orcid":false,"given":"Christopher 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