{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T06:25:42Z","timestamp":1750055142710,"version":"3.37.3"},"reference-count":63,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2016,12,1]],"date-time":"2016-12-01T00:00:00Z","timestamp":1480550400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["VO 718\/6-1"],"award-info":[{"award-number":["VO 718\/6-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["0315828D"],"award-info":[{"award-number":["0315828D"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Neisseria meningitidis<\/jats:named-content>\n            , the meningococcus, bears the potential to cause life-threatening invasive diseases, but it usually colonizes the nasopharynx without causing any symptoms. Within the nasopharynx,\n            <jats:named-content content-type=\"genus-species\">Neisseria meningitidis<\/jats:named-content>\n            must face temperature changes depending on the ambient air temperature. Indeed, the nasopharyngeal temperature can be substantially lower than 37\u00b0C, the temperature commonly used in experimental settings. Here, we compared the levels of meningococcal biofilm formation, autoaggregation, and cellular adherence at 32\u00b0C and 37\u00b0C and found a clear increase in all these phenotypes at 32\u00b0C suggestive of a stronger\n            <jats:italic>in vivo<\/jats:italic>\n            colonization capability at this temperature. A comparative proteome analysis approach revealed differential protein expression levels between 32\u00b0C and 37\u00b0C, predominantly affecting the bacterial envelope. A total of 375 proteins were detected. Use of database annotation or the PSORTb algorithm predicted 49 of those proteins to be localized in the outer membrane, 21 in either the inner or outer membrane, 35 in the periplasm, 56 in the inner membrane, and 208 in the cytosol; for 6 proteins, no annotation or prediction was available. Temperature-dependent regulation of protein expression was seen particularly in the periplasm as well as in the outer and inner membranes.\n            <jats:underline>N<\/jats:underline>\n            eisserial\n            <jats:underline>h<\/jats:underline>\n            eparin\n            <jats:underline>b<\/jats:underline>\n            inding\n            <jats:underline>a<\/jats:underline>\n            ntigen (NHBA), NMB1030, and adhesin complex protein (ACP) showed the strongest upregulation at 32\u00b0C and were partially responsible for the observed temperature-dependent phenotypes. Screening of different global regulators of\n            <jats:named-content content-type=\"genus-species\">Neisseria meningitidis<\/jats:named-content>\n            suggested that the extracytoplasmic sigma factor \u03c3\n            <jats:sup>E<\/jats:sup>\n            might be involved in temperature-dependent biofilm formation. In conclusion, subtle temperature changes trigger adaptation events promoting mucosal colonization by meningococci.\n          <\/jats:p>","DOI":"10.1128\/iai.00584-16","type":"journal-article","created":{"date-parts":[[2016,9,27]],"date-time":"2016-09-27T03:14:22Z","timestamp":1474946062000},"page":"3484-3495","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Impact of Moderate Temperature Changes on Neisseria meningitidis Adhesion Phenotypes and Proteome"],"prefix":"10.1128","volume":"84","author":[{"given":"Martin","family":"Lappann","sequence":"first","affiliation":[{"name":"Institute for Hygiene and Microbiology, University of W\u00fcrzburg, W\u00fcrzburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Otto","sequence":"additional","affiliation":[{"name":"Institute for Microbiology, Ernst Moritz Arndt University, Greifswald, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madita","family":"Brauer","sequence":"additional","affiliation":[{"name":"Institute for Hygiene and Microbiology, University of W\u00fcrzburg, W\u00fcrzburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00f6rte","family":"Becher","sequence":"additional","affiliation":[{"name":"Institute for Microbiology, Ernst Moritz Arndt University, Greifswald, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Vogel","sequence":"additional","affiliation":[{"name":"Institute for Hygiene and Microbiology, University of W\u00fcrzburg, W\u00fcrzburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kay","family":"Johswich","sequence":"additional","affiliation":[{"name":"Institute for Hygiene and Microbiology, University of W\u00fcrzburg, W\u00fcrzburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1078\/1438-4221-00230"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3184\/003685003783238707"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(00)00206-3"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/mas.10031"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(03)00732-0"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200105033441807"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05448.x"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07054.x"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-010-0149-y"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200004000-00021"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1137"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.4.2424-2432.2005"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.7.3621-3626.2002"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.32.2.641-648.1981"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.5.2454-2462.2002"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.4.1194"},{"key":"e_1_3_3_18_2","first-page":"1965","article-title":"Monoclonal antibodies to gonococcal pili: studies on antigenic determinants on pili from variants of strain P9","volume":"129","author":"Virji M","year":"1983","unstructured":"Virji M, Heckels JE, Watt PJ. 1983. 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A novel regulatory switch for increased expression of Neisseria meningitidis NHBA at physiological temperatures found in the human nasopharynx. 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