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Neutrophils and monocytes are myeloid immune cells that play key roles in host defense during pulmonary infection against\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      ; although if not properly regulated, their inflammatory activity can lead to severe lung injury. Thrombospondin-1 (TSP-1) is a host glycoprotein that regulates neutrophil activation in the lungs during\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      infection, protecting the host from mortality. Here, we demonstrate that in addition to regulating the pro-inflammatory phenotype of neutrophils, during\n                      <jats:italic toggle=\"yes\">P. aeruginosa<\/jats:italic>\n                      infection, TSP-1 induces IL-10 in the lung tissue of mice inoculated with this bacterium. Our data show that neutrophils and Ly6C\n                      <jats:sup>+<\/jats:sup>\n                      monocytes are major sources of lung IL-10 during the first 48 h post-infection, a cytokine that is required for host survival, host defense, and to reduce lung inflammation and injury. Further\n                      <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                      studies determined that TSP-1 induces IL-10 production in lipopolysaccharide-stimulated CD11b\n                      <jats:sup>+<\/jats:sup>\n                      Ly6G\n                      <jats:sup>+<\/jats:sup>\n                      Ly6C\n                      <jats:sup>+<\/jats:sup>\n                      cells differentiated from bone marrow precursors through a mechanism dependent on the transcription factor peroxisome proliferator-activated receptor gamma (PPAR\u03b3). Importantly, intratracheal transfer of\n                      <jats:italic toggle=\"yes\">\n                        IL-10\n                        <jats:sup>+\/+<\/jats:sup>\n                      <\/jats:italic>\n                      CD11b\n                      <jats:sup>+<\/jats:sup>\n                      Ly6G\n                      <jats:sup>+<\/jats:sup>\n                      Ly6C\n                      <jats:sup>+<\/jats:sup>\n                      cells 1 day before infection to\n                      <jats:italic toggle=\"yes\">\n                        Thbs1\n                        <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      <\/jats:italic>\n                      mice restored their ability to produce IL-10 in the lungs, improved host defense, and reduced lung inflammation and permeability upon infection. Altogether, our data show that TSP-1 induces IL-10 production in neutrophils and monocytes, enhancing host defense while reducing lung inflammation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/iai.00614-25","type":"journal-article","created":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T14:00:44Z","timestamp":1771941644000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Myeloid-derived interleukin-10 induced by thrombospondin-1 mediates host defense and regulates inflammation during acute bacterial lung infection"],"prefix":"10.1128","volume":"94","author":[{"given":"Agnes","family":"Jara-Collao","sequence":"first","affiliation":[{"name":"Millennium Institute on Immunology and Immunotherapy, Facultad de Ciencias Biol\u00f3gicas, Pontificia Universidad Cat\u00f3lica de Chile","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisca","family":"Rom\u00e1n","sequence":"additional","affiliation":[{"name":"Millennium Institute on Immunology and Immunotherapy, Facultad de Ciencias Biol\u00f3gicas, Pontificia Universidad Cat\u00f3lica de Chile","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gonzalo","family":"Torres-Ayala","sequence":"additional","affiliation":[{"name":"Millennium Institute on Immunology and Immunotherapy, Facultad de Ciencias Biol\u00f3gicas, Pontificia Universidad Cat\u00f3lica de Chile","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Novoa","sequence":"additional","affiliation":[{"name":"Millennium Institute on Immunology and Immunotherapy, Facultad de Ciencias Biol\u00f3gicas, Pontificia Universidad Cat\u00f3lica de Chile","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia","family":"Moreno-Quidecoy","sequence":"additional","affiliation":[{"name":"Millennium Institute on Immunology and Immunotherapy, Facultad de Ciencias Biol\u00f3gicas, Pontificia Universidad Cat\u00f3lica de Chile","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aillairlyn","family":"Jara","sequence":"additional","affiliation":[{"name":"Millennium Institute on Immunology and Immunotherapy, Facultad de Ciencias Biol\u00f3gicas, Pontificia Universidad Cat\u00f3lica de Chile","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7709-6870","authenticated-orcid":true,"given":"Pablo A.","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Millennium Institute on Immunology and Immunotherapy, Facultad de Ciencias Biol\u00f3gicas, Pontificia Universidad Cat\u00f3lica de Chile","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7551-8088","authenticated-orcid":false,"given":"Susan M.","family":"Bueno","sequence":"additional","affiliation":[{"name":"Millennium Institute on Immunology and Immunotherapy, Facultad de Ciencias Biol\u00f3gicas, Pontificia Universidad Cat\u00f3lica de Chile","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexis M.","family":"Kalergis","sequence":"additional","affiliation":[{"name":"Millennium Institute on Immunology and Immunotherapy, Facultad de Ciencias Biol\u00f3gicas, Pontificia Universidad Cat\u00f3lica de Chile","place":["Santiago, Chile"]},{"name":"Departamento de Endocrinolog\u00eda, Facultad de Medicina, Escuela de Medicina, Pontificia Universidad Cat\u00f3lica de Chile","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1593-678X","authenticated-orcid":true,"given":"Hern\u00e1n F.","family":"Pe\u00f1aloza","sequence":"additional","affiliation":[{"name":"Millennium Institute on Immunology and Immunotherapy, Facultad de Ciencias Biol\u00f3gicas, Pontificia Universidad Cat\u00f3lica de Chile","place":["Santiago, Chile"]},{"name":"Departamento de Laboratorios Cl\u00ednicos, Escuela de Medicina, Facultad de Medicina, Pontificia Universidad Cat\u00f3lica de Chile","place":["Santiago, Chile"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.2471\/blt.07.048769"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-021-00259-0"},{"key":"e_1_3_5_4_2","doi-asserted-by":"crossref","unstructured":"OECD. 2023. 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