{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:28:37Z","timestamp":1791404917409,"version":"4.3.3"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Macrophages are multifunctional cells that are active in T<jats:sub>H<\/jats:sub>1- and T<jats:sub>H<\/jats:sub>2-mediated responses. In this study, we demonstrate that human and mouse macrophages collaborate with neutrophils and complement to kill the parasite<jats:italic>Strongyloides stercoralis in vitro<\/jats:italic>. Infection of mice with worms resulted in the induction of alternatively activated macrophages (AAM\u03d5) within the peritoneal cavity. These cells killed the worms<jats:italic>in vivo<\/jats:italic>and collaborated with neutrophils and complement during the<jats:italic>in vitro<\/jats:italic>killing process. AAM\u03d5 generated<jats:italic>in vitro<\/jats:italic>killed larvae more rapidly than naive macrophages, which killed larvae after a longer time period. In contrast, classically activated macrophages were unable to kill larvae either<jats:italic>in vitro<\/jats:italic>or<jats:italic>in vivo<\/jats:italic>. This study adds macrophages to the armamentarium of immune components that function in elimination of parasitic helminths and demonstrate a novel function by which AAM\u03d5 control large extracellular parasites.<\/jats:p>","DOI":"10.1128\/iai.00625-13","type":"journal-article","created":{"date-parts":[[2013,6,25]],"date-time":"2013-06-25T02:00:11Z","timestamp":1372125611000},"page":"3346-3355","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":73,"title":["Human and Mouse Macrophages Collaborate with Neutrophils To Kill Larval Strongyloides stercoralis"],"prefix":"10.1128","volume":"81","author":[{"given":"Sandra","family":"Bonne-Ann\u00e9e","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura A.","family":"Kerepesi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica A.","family":"Hess","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy E.","family":"O'Connell","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James B.","family":"Lok","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, University of Pennsylvania, School of Veterinary Medicine, Philadelphia, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"Nolan","sequence":"additional","affiliation":[{"name":"Department of Pathobiology, University of Pennsylvania, School of Veterinary Medicine, Philadelphia, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Abraham","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.101.4.907"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.crvi.2004.04.005"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2494(99)80039-X"},{"key":"e_1_3_2_5_2","first-page":"149","article-title":"Complement-dependent killing of Nippostrongylus brasiliensis infective larvae by rat alveolar macrophages","volume":"55","author":"Egwang TG","year":"1984","unstructured":"EgwangTGGauldieJBefusD. 1984. Complement-dependent killing of Nippostrongylus brasiliensis infective larvae by rat alveolar macrophages. Clin. Exp. Immunol. 55:149\u2013156.","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2007.76.669"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0931259100"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0602325"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1671"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000813"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/262609"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2008.10.017"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1204351"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2628"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10653"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3071"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.2307\/3283757"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12026-011-8258-2"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1106695"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2011.01.016"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1159\/000233235"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.7.4315"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01958-05"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00931-10"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt822"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1995.1036"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3148.1997.d01-75.x"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1996.0035"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.12.6835-6843.2003"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1089\/107999099314432"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803720"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/173.5.1202"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2009.03.015"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.6.1999"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2008.03.077"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20082048"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201141869"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1451"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2006.10.003"},{"key":"e_1_3_2_40_2","doi-asserted-by":"crossref","first-page":"7302","DOI":"10.4049\/jimmunol.162.12.7302","article-title":"Differences between IL-4- and IL-4 receptor alpha-deficient mice in chronic leishmaniasis reveal a protective role for IL-13 receptor signaling","volume":"162","author":"Mohrs M","year":"1999","unstructured":"MohrsMLedermannBKohlerGDorfmullerAGessnerABrombacherF. 1999. Differences between IL-4- and IL-4 receptor alpha-deficient mice in chronic leishmaniasis reveal a protective role for IL-13 receptor signaling. J. Immunol. 162:7302\u20137308.","journal-title":"J. Immunol."},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2006.05.005"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80477-X"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.7.4948"},{"key":"e_1_3_2_44_2","first-page":"25","article-title":"Strongyloides stercoralis infection","volume":"52","author":"Napier LE","year":"1949","unstructured":"NapierLE. 1949. Strongyloides stercoralis infection. J. Trop. Med. Hyg. 52:25.","journal-title":"J. Trop. Med. Hyg."},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.6.3926"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.05.007"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404034101"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000371"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.2011.01300.x"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-3-7"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-07-442640"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1222878110"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00625-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00625-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,2]],"date-time":"2023-07-02T15:39:34Z","timestamp":1688312374000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00625-13"}},"subtitle":[],"editor":[{"suffix":"Jr.","given":"J. F.","family":"Urban","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,9]]},"references-count":51,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2013,9]]}},"alternative-id":["10.1128\/IAI.00625-13"],"URL":"https:\/\/doi.org\/10.1128\/iai.00625-13","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,9]]},"assertion":[{"value":"2013-05-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-06-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-08-13","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}