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The goal of this work was to identify new potential vaccine candidates through a proteomics approach. We constructed a protein chip array (Antigen Discovery, Inc.) by expressing the open reading frames (ORFs) from<jats:italic>C. trachomatis<\/jats:italic>mouse pneumonitis (MoPn) genomic and plasmid DNA and tested it with serum samples from MoPn-immunized mice. Two groups of BALB\/c female mice were immunized either intranasally or intravaginally with live elementary bodies (EB). Another two groups were immunized by a combination of the intramuscular and subcutaneous routes with UV-treated EB (UV-EB), using either CpG and Montanide as adjuvants to favor a Th1 response or alum to elicit a Th2 response. Serum samples collected at regular intervals postimmunization were tested in the proteome array. The microarray included the expression products of 909 proteins from a total of 921 ORFs of the<jats:italic>Chlamydia<\/jats:italic>MoPn genome and plasmid. A total of 185 immunodominant proteins elicited an early and sustained antibody response in the mice immunized with live EB, and of these, 71 were also recognized by the sera from mice immunized with UV-EB. The reactive antigens included some proteins that were previously described as immunogenic, such as the major outer membrane protein, OmpB, Hsp60, and IncA and proteins from the type III secretion system. In addition, we identified in mice several new immunogens, including 75 hypothetical proteins. In summary, we have identified a new group of immunodominant chlamydial proteins that can be tested for their ability to induce protection.<\/jats:p>","DOI":"10.1128\/iai.00626-10","type":"journal-article","created":{"date-parts":[[2010,10,19]],"date-time":"2010-10-19T03:40:08Z","timestamp":1287459608000},"page":"246-257","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Identification of Immunodominant Antigens by Probing a Whole<i>Chlamydia trachomatis<\/i>Open Reading Frame Proteome Microarray Using Sera from Immunized Mice"],"prefix":"10.1128","volume":"79","author":[{"given":"Maria I.","family":"Cruz-Fisher","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Medical Sciences I, Room D440, University of California, Irvine, Irvine, California 92697-4800"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunmei","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Medical Sciences I, Room D440, University of California, Irvine, Irvine, California 92697-4800"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guifeng","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Medical Sciences I, Room D440, University of California, Irvine, Irvine, California 92697-4800"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sukumar","family":"Pal","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Medical Sciences I, Room D440, University of California, Irvine, Irvine, California 92697-4800"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andy","family":"Teng","sequence":"additional","affiliation":[{"name":"Antigen Discovery, Inc., 1 Technology Drive, Suite E309, Irvine, California 92618"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas M.","family":"Molina","sequence":"additional","affiliation":[{"name":"Antigen Discovery, Inc., 1 Technology Drive, Suite E309, Irvine, California 92618"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew A.","family":"Kayala","sequence":"additional","affiliation":[{"name":"Department of Computer Science, 4038 Bren Hall, University of California, Irvine, Irvine, California 92697-3435"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Vigil","sequence":"additional","affiliation":[{"name":"Department of Medicine, 3052 Hewitt Hall, University of California, Irvine, Irvine, California 92697-4068"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Baldi","sequence":"additional","affiliation":[{"name":"Department of Computer Science, 4038 Bren Hall, University of California, Irvine, Irvine, California 92697-3435"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip L.","family":"Felgner","sequence":"additional","affiliation":[{"name":"Department of Medicine, 3052 Hewitt Hall, University of California, Irvine, Irvine, California 92697-4068"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowu","family":"Liang","sequence":"additional","affiliation":[{"name":"Antigen Discovery, Inc., 1 Technology Drive, Suite E309, Irvine, California 92618"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis M.","family":"de la Maza","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Medical Sciences I, Room D440, University of California, Irvine, Irvine, California 92697-4800"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.6.509"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.05.028"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1551"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.31.3.1161-1176.1981"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.35.3.1024-1031.1982"},{"key":"e_1_3_3_7_2","first-page":"362","volume":"58","year":"2009","unstructured":"Centers for Disease Control and Prevention. 2009. 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