{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T21:59:33Z","timestamp":1780351173557,"version":"3.54.1"},"reference-count":12,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T00:00:00Z","timestamp":1572566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100006492","name":"Division of Intramural Research, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006492","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:p><jats:named-content content-type=\"genus-species\">Giardia lamblia<\/jats:named-content>is usually cultured axenically in TYI-S-33, a complex medium which does not permit survival and growth of mammalian cells. Likewise, medium commonly used to maintain and grow mammalian cells does not support healthy trophozoite survival for more than a few hours. The inability to coculture trophozoites and epithelial cells under optimal conditions limits studies of their interactions as well as interpretation of results.<\/jats:p>","DOI":"10.1128\/iai.00639-19","type":"journal-article","created":{"date-parts":[[2019,8,28]],"date-time":"2019-08-28T05:44:35Z","timestamp":1566971075000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Long-Term Culture of Giardia lamblia in Cell Culture Medium Requires Intimate Association with Viable Mammalian Cells"],"prefix":"10.1128","volume":"87","author":[{"given":"Theodore E.","family":"Nash","sequence":"first","affiliation":[{"name":"Laboratory of Parasitic Diseases, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(83)90120-7"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/mcb.2.4.369"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/152.1.64"},{"key":"e_1_3_2_5_2","first-page":"121","article-title":"Comparison of different isolates of Giardia","volume":"15","author":"Nash T","year":"1985","unstructured":"Nash T. 1985. Comparison of different isolates of Giardia. Microecol Ther 15:121\u2013132.","journal-title":"Microecol Ther"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(92)90119-M"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0081104"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1051\/parasite\/2008153261"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2317"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2018.00244"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(82)80008-5"},{"key":"e_1_3_2_12_2","first-page":"21S","article-title":"Isolating expressed microsporidial genes using a cDNA subtractive hybridization approach","volume":"46","author":"Hayman JR","year":"1999","unstructured":"Hayman JR, Nash TE. 1999. Isolating expressed microsporidial genes using a cDNA subtractive hybridization approach. J Eukaryot Microbiol 46:21S\u201324S.","journal-title":"J Eukaryot Microbiol"},{"key":"e_1_3_2_13_2","doi-asserted-by":"crossref","first-page":"636","DOI":"10.4049\/jimmunol.141.2.636","article-title":"Antigenic variation in Giardia lamblia","volume":"141","author":"Nash TE","year":"1988","unstructured":"Nash TE, Aggarwal A, Adam RD, Conrad JT, Merritt JW, Jr. 1988. Antigenic variation in Giardia lamblia. J Immunol 141:636\u2013641.","journal-title":"J Immunol"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00639-19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00639-19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,19]],"date-time":"2023-09-19T10:12:00Z","timestamp":1695118320000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00639-19"}},"subtitle":[],"editor":[{"given":"De'Broski R.","family":"Herbert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,11]]},"references-count":12,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2019,11]]}},"alternative-id":["10.1128\/IAI.00639-19"],"URL":"https:\/\/doi.org\/10.1128\/iai.00639-19","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11]]},"assertion":[{"value":"2019-08-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e00639-19"}}