{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T03:44:13Z","timestamp":1789703053265,"version":"4.0.1"},"reference-count":68,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2010,2,1]],"date-time":"2010-02-01T00:00:00Z","timestamp":1264982400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Schistosoma mansoni<\/jats:italic>eggs produced by adult worms in the mesenteric vasculature become trapped in the liver, where they induce granulomatous lesions and strong immune responses. Infected individuals suffer from intestinal schistosomiasis (INT) in 90% of cases, whereas the remaining 10% present with severe hepatosplenic schistosomiasis (HS). The CBA\/J mouse model mimics human disease, with 20% of infected mice developing hypersplenomegaly syndrome (HSS) that resembles HS and 80% developing moderate splenomegaly syndrome (MSS) similar to INT. We studied differential patterns of protein expression in livers of 20-week-infected CBA\/J mice with MSS or HSS to understand the molecular changes that underlie these two disease forms. Using differential in-gel electrophoresis to identify differentially expressed protein spots, we found 80 protein spots significantly changed with infection and 35 changes specific to severe disease. In particular, the abundances of prohibitin 2, transferrin isoforms, and major urinary protein isoforms were significantly altered in HSS mice. Furthermore, annexin 5, glutathione<jats:italic>S<\/jats:italic>-transferase pi class, and<jats:italic>S. mansoni<\/jats:italic>phosphoenolpyruvate carboxykinase expression levels changed significantly with schistosome infection. Additionally, levels of major urinary protein decreased and levels of transferrin increased significantly in the sera of HSS mice compared to levels in sera of MSS or control mice, and these differences correlated to the degree of splenomegaly. These findings indicate that the liver protein abundances differ between MSS and HSS mice and may be used for the development of diagnostic markers for the early detection of hepatosplenic schistosomiasis.<\/jats:p>","DOI":"10.1128\/iai.00647-09","type":"journal-article","created":{"date-parts":[[2009,11,24]],"date-time":"2009-11-24T03:58:55Z","timestamp":1259035135000},"page":"618-628","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":16,"title":["Differential Patterns of Liver Proteins in Experimental Murine Hepatosplenic Schistosomiasis"],"prefix":"10.1128","volume":"78","author":[{"given":"B.","family":"Manivannan","sequence":"first","affiliation":[{"name":"Centre for Biodiscovery and School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Rawson","sequence":"additional","affiliation":[{"name":"Centre for Biodiscovery and School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. W.","family":"Jordan","sequence":"additional","affiliation":[{"name":"Centre for Biodiscovery and School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. E.","family":"Secor","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. C.","family":"La Flamme","sequence":"additional","affiliation":[{"name":"Centre for Biodiscovery and School of Biological Sciences, Victoria University of Wellington, Wellington, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M300022-MCP200"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1159\/000096114"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.2307\/3282131"},{"key":"e_1_3_2_5_2","first-page":"23","volume":"16","year":"2004","unstructured":"Arinola, O. G. 2004. Metal binding acute phase proteins and trace elements in Nigerian children with urinary schistosomiasis. Biokemistri16:23-27.","journal-title":"Biokemistri"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.6.3385-3393.2000"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3835(94)90320-4"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200700864"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401194"},{"key":"e_1_3_2_10_2","first-page":"230","volume":"77","year":"1989","unstructured":"Boehme, M. W. J., P. K. Kataaha, and E. J. Holborow. 1989. Autoantibodies to intermediate filaments in sera of patients with Schistosoma mansoni infection. Clin. Exp. Immunol.77:230-233.","journal-title":"Clin. Exp. Immunol."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(97)00098-4"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1022402915431"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1139\/O08-069"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(00)00122-4"},{"key":"e_1_3_2_15_2","first-page":"211","volume":"2","year":"2003","unstructured":"Chu, P., J. Pardo, H. Zhao, C. C. Li, E. Pali, M. M. Shen, K. Qu, S. X. Yu, B. C. B. Huang, P. Yu, E. S. Masuda, S. M. Molineaux, F. Kolbinger, G. Aversa, J. De Vries, D. G. Payan, and X. C. Liao. 2003. Systematic identification of regulatory proteins critical for T-cell activation. J. Biol.2:211-2116.","journal-title":"J. Biol."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.14.6288-6297.2004"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.2307\/3282599"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2744(199607)16:3<123::AID-MC1>3.0.CO;2-I"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/mc.2940020610"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(79)91328-3"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4439(03)00037-1"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(20000601)21:11<2162::AID-ELPS2162>3.0.CO;2-S"},{"key":"e_1_3_2_23_2","doi-asserted-by":"crossref","first-page":"1322","DOI":"10.4049\/jimmunol.146.4.1322","volume":"146","year":"1991","unstructured":"Grzych, J. M., E. Pearce, A. Cheever, Z. A. Caulada, P. Caspar, S. Heiny, F. Lewis, and A. Sher. 1991. Egg deposition is the major stimulus for the production of Th2 cytokines in murine schistomiasis mansoni. J. Immunol.146:1322-1327.","journal-title":"J. Immunol."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-006-9286-4"},{"key":"e_1_3_2_25_2","first-page":"1317","volume":"38","year":"1978","unstructured":"Hammond, K. D., and D. Balinsky. 1978. Activities of key gluconeogenic enzymes and glycogen synthase in rat and human livers, hepatomas, and hepatoma cell cultures. Cancer Res.38:1317-1322.","journal-title":"Cancer Res."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01048-06"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.32.12.1546"},{"key":"e_1_3_2_28_2","first-page":"703","volume":"142","year":"1993","unstructured":"Henderson, G. S., N. A. Nix, M. A. Montesano, D. Gold, G. L. Freeman, Jr., T. L. McCurley, and D. G. Colley. 1993. Two distinct pathological syndromes in male CBA\/J inbred mice with chronic Schistosoma mansoni infections. Am. J. Pathol.142:703-714.","journal-title":"Am. J. Pathol."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200600580"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830270518"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1089\/neu.2008.0562"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200500083"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr050084g"},{"key":"e_1_3_2_34_2","first-page":"227","volume":"13","year":"2002","unstructured":"Krupenko, S. A., and N. V. Oleinik. 2002. 10-Formyltetrahydrofolate dehydrogenase, one of the major folate enzymes, is down-regulated in tumor tissues and possesses suppressor effects on cancer cells. Cell Growth Differ.13:227-236.","journal-title":"Cell Growth Differ."},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.3.1903"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgn198"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M408678200"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2002.6547"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(03)00045-7"},{"key":"e_1_3_2_40_2","unstructured":"Parasitology research & encyclopedic reference of parasitology 2009"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2005.065680"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2005.02.004"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M413343200"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2265.2004.01936.x"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19930101)71:1<36::AID-CNCR2820710107>3.0.CO;2-J"},{"key":"e_1_3_2_46_2","first-page":"124","volume":"24","year":"2005","unstructured":"Otegbayo, J. A., O. G. Arinola, A. Aje, O. A. Oluwasola, O. H. Okiwelu, and L. S. Salimonu. 2005. Usefulness of acute phase proteins for monitoring development of hepatocellular carcinoma in hepatitis B virus carriers. West Afr. J. Med.24:124-127.","journal-title":"West Afr. J. Med."},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12072-008-9113-y"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840050503"},{"key":"e_1_3_2_49_2","first-page":"25","volume":"16","year":"2006","unstructured":"Rizk, M., T. A. Fayed, M. Badawy, and N. S. El-Regal. 2006. Effect of different durations of Schistosoma mansoni infection on the levels of some antioxidants in mice. Med. J. Islam. World Acad. Sci.16:25-34.","journal-title":"Med. J. Islam. World Acad. Sci."},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.291.5512.2370"},{"key":"e_1_3_2_51_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_52_2","first-page":"731","volume":"60","year":"1977","unstructured":"Saif, M., J. Tawfik, M. A. Ali, S. F. El-Mahrouky, N. Galil, and M. N. Galal. 1977. Serum transferrin, albumin and IgG levels in hepatosplenic bilharziasis in Egypt. J. Egypt. Med. Assoc.60:731-743.","journal-title":"J. Egypt. Med. Assoc."},{"key":"e_1_3_2_53_2","first-page":"103","volume":"7","year":"2004","unstructured":"Salawu, L., and O. G. Arinola. 2004. Acute phase proteins in pregnant women with urinary schistosomiasis in live villages, Osun State, Nigeria. Afr. J. Biomed. Res.7:103-106.","journal-title":"Afr. J. Biomed. Res."},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840200326"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M702212200"},{"key":"e_1_3_2_56_2","first-page":"131","volume":"55","year":"2008","unstructured":"Schi\u00f8dt, F. V. 2008. Gc-globulin in liver disease. Dan. Med. Bull.55:131-146.","journal-title":"Dan. Med. Bull."},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(99)80089-6"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200600983"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-008-0431-x"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M600449-MCP200"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200300552"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780456"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(03)00029-9"},{"key":"e_1_3_2_64_2","first-page":"1508","year":"1977","unstructured":"Van Waes, L., and C. S. Lieber. 1977. Glutamate dehydrogenase: a reliable marker of liver cell necrosis in the alcoholic. Br. Med. J.ii:1508-1510.","journal-title":"Br. Med. J."},{"key":"e_1_3_2_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-2797(00)00211-8"},{"key":"e_1_3_2_66_2","first-page":"576","year":"2008","unstructured":"Virtanen, T., and T. Kinnunen. 2008. Clinical allergy and immunology series, p. 576. In R. F. Lockey and D. K. Ledford (ed.), Allergens and allergen immunotherapy, 4th ed. Informa Health Care, New York, NY.","journal-title":"Allergens and allergen immunotherapy"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2005.11.001"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2004.00176.x"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2003.06.068"}],"container-title":["Infection and Immunity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00647-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/IAI.00647-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,27]],"date-time":"2023-05-27T17:06:32Z","timestamp":1685207192000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/IAI.00647-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,2]]},"references-count":68,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2010,2]]}},"alternative-id":["10.1128\/IAI.00647-09"],"URL":"https:\/\/doi.org\/10.1128\/iai.00647-09","relation":{},"ISSN":["0019-9567","1098-5522"],"issn-type":[{"value":"0019-9567","type":"print"},{"value":"1098-5522","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,2]]},"assertion":[{"value":"2009-06-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-11-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}