{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T15:30:41Z","timestamp":1771515041493,"version":"3.50.1"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2011,1,1]],"date-time":"2011-01-01T00:00:00Z","timestamp":1293840000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In the enteric pathogen\n            <jats:italic>Vibrio cholerae<\/jats:italic>\n            , expression of the major virulence factors is controlled by the hierarchical expression of several regulatory proteins comprising the ToxR regulon. In this study, we demonstrate that disruption of the\n            <jats:italic>fadD<\/jats:italic>\n            gene encoding a long-chain fatty acyl coenzyme A ligase has marked effects on expression of the ToxR virulence regulon, motility, and\n            <jats:italic>in vivo<\/jats:italic>\n            lethality of\n            <jats:italic>V. cholerae<\/jats:italic>\n            . In the\n            <jats:italic>V. cholerae fadD<\/jats:italic>\n            mutant, expression of the major virulence genes\n            <jats:italic>ctxAB<\/jats:italic>\n            and\n            <jats:italic>tcpA<\/jats:italic>\n            , encoding cholera toxin (CT), and the major subunit of the toxin-coregulated pilus (TCP) was drastically repressed and a growth-phase-dependent reduction in the expression of\n            <jats:italic>toxT<\/jats:italic>\n            , encoding the transcriptional activator of\n            <jats:italic>ctxAB<\/jats:italic>\n            and\n            <jats:italic>tcpA<\/jats:italic>\n            , was observed. Expression of\n            <jats:italic>toxT<\/jats:italic>\n            from an inducible promoter completely restored CT to wild-type levels in the\n            <jats:italic>V. cholerae fadD<\/jats:italic>\n            mutant, suggesting that FadD probably acts upstream of\n            <jats:italic>toxT<\/jats:italic>\n            expression. Expression of\n            <jats:italic>toxT<\/jats:italic>\n            is activated by the synergistic effect of two transcriptional regulators, TcpP and ToxR. Reverse transcription-PCR and Western blot analysis indicated that although gene expression and production of both TcpP and ToxR are unaffected in the\n            <jats:italic>fadD<\/jats:italic>\n            mutant strain, membrane localization of TcpP, but not ToxR, is severely impaired in the\n            <jats:italic>fadD<\/jats:italic>\n            mutant strain from the mid-logarithmic phase of growth. Since the decrease in\n            <jats:italic>toxT<\/jats:italic>\n            expression occurred concomitantly with the reduction in membrane localization of TcpP, a direct correlation between the defect in membrane localization of TcpP and reduced\n            <jats:italic>toxT<\/jats:italic>\n            expression in the\n            <jats:italic>fadD<\/jats:italic>\n            mutant strain is suggested.\n          <\/jats:p>","DOI":"10.1128\/iai.00663-10","type":"journal-article","created":{"date-parts":[[2010,11,2]],"date-time":"2010-11-02T03:17:34Z","timestamp":1288667854000},"page":"258-266","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["A\n            <i>fadD<\/i>\n            mutant of\n            <i>Vibrio cholerae<\/i>\n            Is Impaired in the Production of Virulence Factors and Membrane Localization of the Virulence Regulatory Protein TcpP"],"prefix":"10.1128","volume":"79","author":[{"given":"Sreejana","family":"Ray","sequence":"first","affiliation":[{"name":"Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, Council of Scientific and Industrial Research, Kolkata 700 032, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Epshita","family":"Chatterjee","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, Council of Scientific and Industrial Research, Kolkata 700 032, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arpita","family":"Chatterjee","sequence":"additional","affiliation":[{"name":"Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, Council of Scientific and Industrial Research, Kolkata 700 032, 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