{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,14]],"date-time":"2026-03-14T10:35:36Z","timestamp":1773484536445,"version":"3.50.1"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Entamoeba histolytica<\/jats:italic>\n            is the agent of amebic colitis. In this work we examined the intestinal NF-\u03baB response to this parasite. Using an enzyme-linked immunosorbent assay (ELISA) and an electrophoretic mobility shift assay, we found that the NF-\u03baB subunit p50 predominated in nuclear extracts of whole cecal tissue and of isolated crypts from mice inoculated with\n            <jats:italic>E. histolytica<\/jats:italic>\n            . p50 was protective, since C57BL\/6 and 129 mice in which there was targeted deletion of this subunit were more susceptible to\n            <jats:italic>E. histolytica<\/jats:italic>\n            infection as measured by culture results, cecal parasite ELISA results, and\/or histologic scores. The transepithelial electrical resistance of cecal explants from C57BL\/6 and 129 p50 knockout mice decreased markedly in response to the parasite compared with the transepithelial electrical resistance of their wild-type counterparts, suggesting that a protective function of p50 was present in the epithelium itself. This work shows that NF-\u03baB activity, particularly activity of the p50 subunit, is one factor that contributes to resistance of the gut to\n            <jats:italic>E. histolytica<\/jats:italic>\n            infection.\n          <\/jats:p>","DOI":"10.1128\/iai.00669-09","type":"journal-article","created":{"date-parts":[[2010,1,20]],"date-time":"2010-01-20T04:52:47Z","timestamp":1263963167000},"page":"1475-1481","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["The NF-\u03baB p50 Subunit Is Protective during Intestinal\n            <i>Entamoeba histolytica<\/i>\n            Infection of 129 and C57BL\/6 Mice"],"prefix":"10.1128","volume":"78","author":[{"given":"Kyou-Nam","family":"Cho","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia 22908"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen M.","family":"Becker","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia 22908"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric R.","family":"Houpt","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia 22908"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.8.4481"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.8.4522-4529.2005"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2005.07.057"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/376167a0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.10.4745-4750.2003"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00736-08"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.3.1443"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.2.1116-1125.2004"},{"key":"e_1_3_2_10_2","first-page":"669","volume":"72","year":"1987","unstructured":"Gathiram, V., and T. 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