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Among the relatively few regulators of adaptive gene expression present in the borrelial genome is an open reading frame (ORF), BB0184, annotated as CsrA (<jats:italic>c<\/jats:italic>arbon<jats:italic>s<\/jats:italic>torage<jats:italic>r<\/jats:italic>egulator<jats:italic>A<\/jats:italic>). CsrA, in several bacterial species, has been characterized as a small RNA binding protein that functions as a global regulator affecting mRNA stability or levels of translation of multiple ORFs. Consistent with known functions of CsrA, overexpression of CsrA from<jats:italic>B. burgdorferi<\/jats:italic>(CsrA<jats:sub>Bb<\/jats:sub>) in<jats:italic>Escherichia coli<\/jats:italic>resulted in reduced accumulation of glycogen. We determined that<jats:italic>csrA<\/jats:italic><jats:sub>Bb<\/jats:sub>is part of the<jats:italic>flgK<\/jats:italic>motility operon and that the synthesis of CsrA<jats:sub>Bb<\/jats:sub>was increased when<jats:italic>B. burgdorferi<\/jats:italic>was propagated under fed-tick conditions. Overexpression of CsrA<jats:sub>Bb<\/jats:sub>in<jats:italic>B. burgdorferi<\/jats:italic>strain B31 (ML23, lp25-negative clonal isolate) resulted in a clone, designated ES25, which exhibited alterations in colony morphology and a significant reduction in the levels of FlaB. Several lipoproteins previously characterized as playing a role in infectivity were also altered in ES25. Real-time reverse transcription-PCR analysis of RNA revealed significant differences in the transcriptional levels of<jats:italic>ospC<\/jats:italic>in ES25, while there were no such differences in the levels of other transcripts, suggesting posttranscriptional regulation of expression of these latter genes. These observations indicate that CsrA<jats:sub>Bb<\/jats:sub>plays a role in the regulation of expression of pathophysiological determinants of<jats:italic>B. burgdorferi<\/jats:italic>, and further characterization of CsrA<jats:sub>Bb<\/jats:sub>will help in better understanding of the regulators of gene expression in<jats:italic>B. burgdorferi.<\/jats:italic><\/jats:p>","DOI":"10.1128\/iai.00673-09","type":"journal-article","created":{"date-parts":[[2009,9,9]],"date-time":"2009-09-09T01:33:13Z","timestamp":1252459993000},"page":"5149-5162","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Overexpression of CsrA (BB0184) Alters the Morphology and Antigen Profiles of<i>Borrelia burgdorferi<\/i>"],"prefix":"10.1128","volume":"77","author":[{"given":"Eva","family":"Sanjuan","sequence":"first","affiliation":[{"name":"South Texas Center for Emerging Infectious Diseases and Department of Biology, The University 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