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We have recently generated monoclonal antibodies (MAbs) to an<jats:italic>H. capsulatum<\/jats:italic>surface-expressed heat shock protein of 60 kDa (Hsp60) that modify disease in a murine histoplasmosis model. Interestingly, the MAbs induced different degrees of yeast cell agglutination<jats:italic>in vitro<\/jats:italic>. In the present study, we characterized the agglutination effects of the antibodies to Hsp60 on<jats:italic>H. capsulatum<\/jats:italic>yeast cells by light microscopy, flow cytometry, dynamic light scattering, measuring zeta potential, and using optical tweezers. We found that immunoglobulin Gs (IgGs) to Hsp60 cause<jats:italic>H. capsulatum<\/jats:italic>aggregation dependent on the (i) concentration of MAbs, (ii) MAb binding constant, and (iii) IgG subclass. Furthermore, infection of macrophages using agglutinates of various sizes after incubation with different Hsp60-binding MAbs induced association to macrophages through distinct cellular receptors and differentially affected macrophage antifungal functions. Hence, the capacity of IgG MAbs to agglutinate<jats:italic>H. capsulatum<\/jats:italic>significantly impacted pathogenic mechanisms of<jats:italic>H. capsulatum<\/jats:italic>during macrophage infection, and the effect was dependent on the antibody subclass and antigen epitope.<\/jats:p>","DOI":"10.1128\/iai.00673-10","type":"journal-article","created":{"date-parts":[[2010,12,7]],"date-time":"2010-12-07T06:20:46Z","timestamp":1291702846000},"page":"918-927","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Agglutination of<i>Histoplasma capsulatum<\/i>by IgG Monoclonal Antibodies against Hsp60 Impacts Macrophage Effector Functions"],"prefix":"10.1128","volume":"79","author":[{"given":"Allan Jefferson","family":"Guimara\u0303es","sequence":"first","affiliation":[{"name":"Department of Medicine (Division of Infectious Diseases), Albert Einstein College of Medicine of Yeshiva University, Bronx, New York"},{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susana","family":"Frases","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Biotecnologia (LABIO), Instituto Nacional de Metrologia, Normalizac\u0327a\u0303o e Qualidade Industrial (INMETRO), Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno","family":"Pontes","sequence":"additional","affiliation":[{"name":"LPO-COPEA, Instituto de Cie\u0302ncias Biome\u0301dicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana Duarte","family":"de Cerqueira","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Estudos Integrados em Biologia Microbiana, Instituto de Microbiologia Professor Paulo de Go\u0301es, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcio L.","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Estudos Integrados em Biologia Microbiana, Instituto de Microbiologia Professor Paulo de Go\u0301es, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan Bessa","family":"Viana","sequence":"additional","affiliation":[{"name":"LPO-COPEA, Instituto de Cie\u0302ncias Biome\u0301dicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"},{"name":"Instituto de Fi\u0301sica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonardo","family":"Nimrichter","sequence":"additional","affiliation":[{"name":"Laborato\u0301rio de Estudos Integrados em Biologia Microbiana, Instituto de Microbiologia Professor Paulo de Go\u0301es, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joshua Daniel","family":"Nosanchuk","sequence":"additional","affiliation":[{"name":"Department of Medicine (Division of Infectious Diseases), Albert Einstein College of Medicine of Yeshiva University, Bronx, New York"},{"name":"Department of Microbiology and Immunology, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/513989"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5321.2024"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1309\/MH5B-GAQ2-KY19-FT7P"},{"key":"e_1_3_2_5_2","doi-asserted-by":"crossref","first-page":"2260","DOI":"10.4049\/jimmunol.112.6.2260","volume":"112","year":"1974","unstructured":"Diamond, R. 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