{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T03:34:13Z","timestamp":1791171253274,"version":"4.1.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2007,11,1]],"date-time":"2007-11-01T00:00:00Z","timestamp":1193875200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Salmonella<\/jats:italic>serovars contain a wide variety of putative fimbrial systems that may contribute to colonization of specific niches.<jats:italic>Salmonella enterica<\/jats:italic>serovar Typhi is the etiologic agent of typhoid fever and is a pathogen specific to humans. In a previous study, we identified a gene, STY3920 (<jats:italic>stgC<\/jats:italic>), encoding the predicted usher of the<jats:italic>stg<\/jats:italic>fimbrial operon, that was expressed by serovar Typhi during infection of human macrophages. The<jats:italic>stg<\/jats:italic>genes are located in the<jats:italic>glmS<\/jats:italic>-<jats:italic>pstS<\/jats:italic>intergenic region in serovar Typhi and certain<jats:italic>Escherichia coli<\/jats:italic>strains, but they are absent in other<jats:italic>S. enterica<\/jats:italic>serovars. We cloned the<jats:italic>stg<\/jats:italic>fimbrial operon into a nonfimbriate<jats:italic>E. coli<\/jats:italic>K-12 strain and into<jats:italic>S. enterica<\/jats:italic>serovar Typhimurium. We demonstrated that the<jats:italic>stg<\/jats:italic>fimbrial operon contributed to increased adherence to human epithelial cells. Transcriptional fusion assays with serovar Typhi suggested that<jats:italic>stg<\/jats:italic>is preferentially expressed in minimal medium. Deletion of<jats:italic>stg<\/jats:italic>reduced adherence of serovar Typhi to epithelial cells. However, deletion of<jats:italic>stg<\/jats:italic>increased uptake of serovar Typhi by human macrophages, and overexpression of<jats:italic>stg<\/jats:italic>in serovar Typhi and serovar Typhimurium strains reduced phagocytosis by human macrophages. These strains survived inside macrophages as well as the wild-type parent. Although the<jats:italic>stgC<\/jats:italic>gene contains a premature stop codon that disrupts the expected open reading frame encoding the usher and is therefore considered a pseudogene, our results show that the<jats:italic>stg<\/jats:italic>operon may encode a functional fimbria. Thus, this serovar Typhi-specific fimbrial operon contributes to interactions with host cells, and further characterization is important for understanding the role of the<jats:italic>stg<\/jats:italic>fimbrial cluster in typhoid fever pathogenesis.<\/jats:p>","DOI":"10.1128\/iai.00674-07","type":"journal-article","created":{"date-parts":[[2007,8,21]],"date-time":"2007-08-21T00:58:49Z","timestamp":1187657929000},"page":"5264-5271","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Contribution of the<i>stg<\/i>Fimbrial Operon of<i>Salmonella enterica<\/i>Serovar Typhi during Interaction with Human Cells"],"prefix":"10.1128","volume":"75","author":[{"given":"Chantal","family":"Forest","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Montreal, C.P. 6128 Succursale Centre-Ville, Montre\u0301al, Que\u0301bec, Canada H3C 3J7"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se\u0301bastien P.","family":"Faucher","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Montreal, C.P. 6128 Succursale Centre-Ville, Montre\u0301al, Que\u0301bec, Canada H3C 3J7"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Poirier","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Montreal, C.P. 6128 Succursale Centre-Ville, Montre\u0301al, Que\u0301bec, Canada H3C 3J7"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Se\u0301bastien","family":"Houle","sequence":"additional","affiliation":[{"name":"INRS-Institut Armand-Frappier, 531 boul. des Prairies, Laval, Que\u0301bec, Canada H7V 1B7"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles M.","family":"Dozois","sequence":"additional","affiliation":[{"name":"INRS-Institut Armand-Frappier, 531 boul. des Prairies, Laval, Que\u0301bec, Canada H7V 1B7"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"France","family":"Daigle","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Montreal, C.P. 6128 Succursale Centre-Ville, Montre\u0301al, Que\u0301bec, Canada H3C 3J7"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1996.d01-1718.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(96)00560-4"},{"key":"e_1_3_2_4_2","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1046\/j.1365-2958.2001.02593.x","volume":"41","year":"2001","unstructured":"Daigle, F., J. 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