{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T08:32:53Z","timestamp":1773131573531,"version":"3.50.1"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2008,12,1]],"date-time":"2008-12-01T00:00:00Z","timestamp":1228089600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The lipooligosaccharide (LOS) of\n            <jats:italic>Neisseria meningitidis<\/jats:italic>\n            can be decorated with phosphoethanolamine (PEA) at the 4\u2032 position of lipid A and at the O-3 and O-6 positions of the inner core of the heptose II residue. The biological role of PEA modification in\n            <jats:italic>N. meningitidis<\/jats:italic>\n            remains unclear. During the course of our studies to elucidate the pathogenicity of the ST-2032 (invasive) meningococcal clonal group, disruption of\n            <jats:italic>lptA<\/jats:italic>\n            , the gene that encodes the PEA transferase for 4\u2032 lipid A, led to a approximately 10-fold decrease in\n            <jats:italic>N. meningitidis<\/jats:italic>\n            adhesion to four kinds of human endothelial and epithelial cell lines at an multiplicity of infection of 5,000. Complementation of the\n            <jats:italic>lptA<\/jats:italic>\n            gene in a \u0394\n            <jats:italic>lptA<\/jats:italic>\n            mutant restored wild-type adherence. By matrix-assisted laser desorption ionization-time-of-flight mass spectrometry analysis, PEA was lost from the lipid A of the \u0394\n            <jats:italic>lptA<\/jats:italic>\n            mutant compared to that of the wild-type strain. The effect of LptA on meningococcal adhesion was independent of other adhesins such as pili, Opc, Opa, and PilC but was inhibited by the presence of capsule. These results indicate that modification of LOS with PEA by LptA enhances meningococcal adhesion to human endothelial and epithelial cells in unencapsulated\n            <jats:italic>N. meningitidis<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/iai.00676-08","type":"journal-article","created":{"date-parts":[[2008,9,30]],"date-time":"2008-09-30T00:44:51Z","timestamp":1222735491000},"page":"5777-5789","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Modification of Lipooligosaccharide with Phosphoethanolamine by LptA in\n            <i>Neisseria meningitidis<\/i>\n            Enhances Meningococcal Adhesion to Human Endothelial and Epithelial Cells"],"prefix":"10.1128","volume":"76","author":[{"given":"Hideyuki","family":"Takahashi","sequence":"first","affiliation":[{"name":"Department of Bacteriology, National Institute of Infectious Diseases, Tokyo, 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