{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T02:36:57Z","timestamp":1792550217346,"version":"4.5.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">Porphyromonas gingivalis<\/jats:named-content>\n            is a Gram-negative obligately anaerobic bacterium associated with several forms of periodontal disease, most closely with chronic periodontitis. Previous studies demonstrated that OxyR plays an important role in the aerotolerance of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. gingivalis<\/jats:named-content>\n            by upregulating the expression of oxidative-stress genes. Increases in oxygen tension and in H\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>2<\/jats:sub>\n            both induce activation of OxyR. It is also known that\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. gingivalis<\/jats:named-content>\n            requires hemin as an iron source for its growth. In this study, we found that a hemin-limited growth environment significantly enhanced OxyR activity in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. gingivalis<\/jats:named-content>\n            . As a result, expression of\n            <jats:italic>sod<\/jats:italic>\n            ,\n            <jats:italic>dps<\/jats:italic>\n            , and\n            <jats:italic>ahpC<\/jats:italic>\n            was also upregulated. Using a chromatin immunoprecipitation quantitative PCR (qPCR) analysis, DNA binding of activated OxyR to the promoter of the\n            <jats:italic>sod<\/jats:italic>\n            gene was enhanced in\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. gingivalis<\/jats:named-content>\n            grown under hemin-limited conditions compared to excess-hemin conditions. Cellular tolerance of H\n            <jats:sub>2<\/jats:sub>\n            O\n            <jats:sub>2<\/jats:sub>\n            was also enhanced when hemin was limited in the growth medium of\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. gingivalis<\/jats:named-content>\n            . Our work supports a model in which hemin serves as a signal for the regulation of OxyR activity and indicates that\n            <jats:named-content xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" content-type=\"genus-species\" xlink:type=\"simple\">P. gingivalis<\/jats:named-content>\n            coordinately regulates expression of oxidative-stress-related genes by this hemin concentration-dependent pathway.\n          <\/jats:p>","DOI":"10.1128\/iai.00680-12","type":"journal-article","created":{"date-parts":[[2012,7,24]],"date-time":"2012-07-24T04:22:20Z","timestamp":1343103740000},"page":"3471-3480","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["OxyR Activation in Porphyromonas gingivalis in Response to a Hemin-Limited Environment"],"prefix":"10.1128","volume":"80","author":[{"given":"Hua","family":"Xie","sequence":"first","affiliation":[{"name":"School of Dentistry, Meharry Medical College, Nashville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cunge","family":"Zheng","sequence":"additional","affiliation":[{"name":"School of Dentistry, Meharry Medical College, Nashville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.5.1415-1428.1999"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-6445(03)00055-X"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.11.6161"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2010.07420.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1990.tb02341.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01270-08"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.7.2454-2462.2006"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.55.4.561-585.1991"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.163535"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.05493-11"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2009.00329.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.7.3367-3375.1999"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2007.00429.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03015.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01014-10"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.18.5591-5601.2003"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04291.x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.28537-0"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsre.2004.09.001"},{"key":"e_1_3_2_21_2","first-page":"167","article-title":"In vitro recombination and mutagenesis by overlap extension PCR","volume":"57","author":"Pogulis RJ","year":"1996","unstructured":"PogulisRJVallejoANPeaseLR. 1996. 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