{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T18:28:15Z","timestamp":1790533695229,"version":"4.1.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2007,11,1]],"date-time":"2007-11-01T00:00:00Z","timestamp":1193875200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Infect Immun"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Brucella<\/jats:italic>\n            strains are facultative intracellular pathogens that induce chronic diseases in humans and animals. This observation implies that\n            <jats:italic>Brucella<\/jats:italic>\n            subverts innate and specific immune responses of the host to develop its full virulence. Deciphering the genes involved in the subversion of the immune system is of primary importance for understanding the virulence of the bacteria, for understanding the pathogenic consequences of infection, and for designing an efficient vaccine. We have developed an in vitro system involving human macrophages infected by\n            <jats:italic>Brucella suis<\/jats:italic>\n            and activated syngeneic \u03b39\u03b42 T lymphocytes. Under these conditions, multiplication of\n            <jats:italic>B. suis<\/jats:italic>\n            inside macrophages is only slightly reduced. To identify the genes responsible for this reduced sensitivity, we screened a library of 2,000 clones of transposon-mutated\n            <jats:italic>B. suis<\/jats:italic>\n            . For rapid and quantitative analysis of the multiplication of the bacteria, we describe a simple method based on Alamar blue reduction, which is compatible with screening a large library. By comparing multiplication inside macrophages alone and multiplication inside macrophages with activated \u03b39\u03b42 T cells, we identified four genes of\n            <jats:italic>B. suis<\/jats:italic>\n            that were necessary to resist to the action of the \u03b39\u03b42 T cells. The putative functions of these genes are discussed in order to propose possible explanations for understanding their exact role in the subversion of innate immunity.\n          <\/jats:p>","DOI":"10.1128\/iai.00690-07","type":"journal-article","created":{"date-parts":[[2007,8,21]],"date-time":"2007-08-21T00:58:49Z","timestamp":1187657929000},"page":"5167-5174","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Identification and Isolation of\n            <i>Brucella suis<\/i>\n            Virulence Genes Involved in Resistance to the Human Innate Immune System"],"prefix":"10.1128","volume":"75","author":[{"given":"Janny","family":"Liautard","sequence":"first","affiliation":[{"name":"CNRS-UMR 5236 and Universite\u0301 Montpellier 2, place Euge\u0300ne Bataillon, 34000 Montpellier, 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